Editors:
- Readily reproducible techniques for synthesizing functional biomolecular reagents
- Emphasis on the interplay of advanced organic synthesis, molecular biology, surface biotechnology and biomaterials engineering
- Step-by-step laboratory protocols for the preparation of protein, nucleic acid, glycosyl and lipid conjugates
- Applications ranging from novel biomedical diagnostics to therapeutics and biomaterials
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 751)
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Table of contents (36 protocols)
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Front Matter
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Protein Conjugates
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Front Matter
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Nucleic Acid Conjugates
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Front Matter
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Glycosyl and Lipid Conjugates
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Front Matter
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About this book
Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology. In this second edition of Bioconjugation Protocols: Strategies and Methods, expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience/engineering. These readily reproducible methods cover the preparation of biomolecular conjugates using a variety of labeling techniques and semisynthetic approaches. Additional chapters address the biofunctionalization of surface structures, including organic/inorganic thin films, as well as various types of nanostructures (magnetic nanoparticles, quantum dots, carbon nanotubes, and silicon nanowire devices). All the protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods, Second Edition offers both novice and experienced researchers access to the broad array of techniques needed to carry out the semisynthesis of functional biomolecular reagents and/or the biofunctionalization of surfaces and structures of unique interest for a widevariety of applications, ranging from novel biomedical diagnostics to powerful new therapeutics to advanced biomaterials.
Editors and Affiliations
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Pacific Biosciences, Menlo Park, USA
Sonny S. Mark
Bibliographic Information
Book Title: Bioconjugation Protocols
Book Subtitle: Strategies and Methods
Editors: Sonny S. Mark
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-151-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-150-5Published: 23 June 2011
Softcover ISBN: 978-1-4939-6096-5Published: 23 August 2016
eBook ISBN: 978-1-61779-151-2Published: 15 June 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XVI, 605
Number of Illustrations: 182 b/w illustrations
Topics: Biochemistry, general, Biomaterials, Biomedical Engineering and Bioengineering