MicroRNAs in Development

Methods and Protocols

  • Tamas Dalmay

Part of the Methods in Molecular Biology book series (MIMB, volume 732)

Table of contents

  1. Front Matter
    Pages i-x
  2. Éva Várallyay, Zoltán Havelda
    Pages 9-23
  3. Sara López-Gomollón
    Pages 25-38
  4. Nana Jacobsen, Ditte Andreasen, Peter Mouritzen
    Pages 39-54
  5. Xiaoqing Tang, Xiaohu Tang, Jozsef Gal, Natasha Kyprianou, Haining Zhu, Guiliang Tang
    Pages 69-88
  6. Pablo Andrés Manavella, Ignacio Rubio-Somoza
    Pages 121-130
  7. Ignacio Rubio-Somoza, Pablo Andrés Manavella
    Pages 131-137
  8. Dimitrios G. Zisoulis, Gene W. Yeo, Amy E. Pasquinelli
    Pages 169-185
  9. César Llave, José Manuel Franco-Zorrilla, Roberto Solano, Daniel Barajas
    Pages 187-208
  10. Back Matter
    Pages 225-227

About this book


Since the discovery of microRNAs, developmental biologists have striven to understand the role of miRNAs in development and disease. MicroRNAs in Development: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression, followed by protocols to manipulate the activity of miRNAs in various organisms, and it concludes with a section that outlines different methods to identify and validate miRNA targets in animals and plants. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, MicroRNAs in Development: Methods and Protocols serves as a practical guide for scientists of all backgrounds and conveys the appropriate sense of fascination associated with this vital field of research.


Detection techniques Functional development Plant and animal models Profiling Target identification miRNA

Editors and affiliations

  • Tamas Dalmay
    • 1
  1. 1.School of Biological SciencesUniversity of East AngliaNorwichUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-082-9
  • Online ISBN 978-1-61779-083-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site