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Serum/Plasma Proteomics

Methods and Protocols

  • Richard J. Simpson
  • David W. Greening

Part of the Methods in Molecular Biology book series (MIMB, volume 728)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Fractionation Strategies for In-depth Blood Proteome Analysis

    1. Front Matter
      Pages 1-1
    2. Lynn A. Beer, Hsin-Yao Tang, Kurt T. Barnhart, David W. Speicher
      Pages 3-27
    3. Simon Sheng, Helena Skalnikova, Andrew Meng, John Tra, Qin Fu, Allen Everett et al.
      Pages 29-46
    4. Hsin-Yao Tang, Lynn A. Beer, David W. Speicher
      Pages 47-67
    5. Maryann S. Vogelsang, Kian Kani, Jonathan E. Katz, Parag Mallick
      Pages 87-107
    6. David W. Greening, Richard J. Simpson
      Pages 109-124
  3. Blood Collection and Handling Strategies

    1. Front Matter
      Pages 135-135
    2. Harald Tammen, Rüdiger Hess
      Pages 151-159
    3. Jizu Yi, Zhaoxia Liu, Craig A. Gelfand, David Craft
      Pages 161-175
  4. Methods for Quantitative Assaying of Blood-Based Biomarkers

    1. Front Matter
      Pages 177-177
    2. Reto Ossola, Ralph Schiess, Paola Picotti, Oliver Rinner, Lukas Reiter, Ruedi Aebersold
      Pages 179-194
    3. Oliver K. Bernhard, Rommel A. Mathias, Thomas W. Barnes, Richard J. Simpson
      Pages 195-206
    4. Vathany Kulasingam, Christopher R. Smith, Ihor Batruch, Eleftherios P. Diamandis
      Pages 207-218
    5. Feliciano Priego-Capote, Maria Ramírez-Boo, Denis Hochstrasser, Jean-Charles Sanchez
      Pages 219-232
  5. Proteome Analysis of Blood Cell Components, Vesicles, and Blood-Related Fluids

    1. Front Matter
      Pages 233-233
    2. Douglas D. Taylor, Wolfgang Zacharias, Cicek Gercel-Taylor
      Pages 235-246
    3. Wei Sun, Ying Jiang, Fuchu He
      Pages 247-257
    4. Rosemary L. Sparrow, David W. Greening, Richard J. Simpson
      Pages 259-265
    5. David W. Greening, Rosemary L. Sparrow, Richard J. Simpson
      Pages 267-278
    6. Thomas Premsler, Urs Lewandrowski, Albert Sickmann, René Peiman Zahedi
      Pages 279-290
  6. Bioinformatic Analysis of Blood Proteins and Peptides

    1. Front Matter
      Pages 291-291
    2. Timothy Clough, Siegmund Braun, Vladimir Fokin, Ilka Ott, Susanne Ragg, Gunther Schadow et al.
      Pages 293-319
    3. Joseph M. Foster, Lennart Martens
      Pages 333-347
    4. Terry Farrah, Eric W. Deutsch, Ruedi Aebersold
      Pages 349-374
  7. Back Matter
    Pages 375-397

About this book

Introduction

Blood science has become a cornerstone of multiple disciplines, including clinical chemistry, disease diagnosis, and therapeutic monitoring.  Over the past decade, we have witnessed the advent of increasingly powerful proteomics technologies that allow greater fundamental insights into the blood proteome.  These technological improvements have, in part, fuelled the quest for the discovery of novel blood-based biomarkers of disease. Serum/Plasma Proteomics: Methods and Protocols is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, this detailed volume covers fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays,  proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, and finally data management, statistical design, and bioinformatic challenges. This book, contributed to by leading experts in the field, provides a valuable foundation for the development and application of blood-based proteomics. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Serum/Plasma Proteomics: Methods and Protocols, with its well-honed methodologies, seeks to serve both professionals and investigators new to the field in an effort to further our knowledge of this fundamental science.

Keywords

Blood Proteomics Blood-based Biomarkers Hematology Pathology Therapeutic Monitoring

Editors and affiliations

  • Richard J. Simpson
    • 1
  • David W. Greening
    • 2
  1. 1.Ludwig Inst. for Cancer Research, Joint Proteomics Laboratory (JPSL)Royal Melbourne HospitalParkvilleAustralia
  2. 2.Ludwig Inst. for Cancer Research, Joint Proteomics LaboratoryRoyal Melbourne HospitalParkvilleAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-068-3
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-067-6
  • Online ISBN 978-1-61779-068-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site