Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Anthony E. Pegg, Robert A. Casero Jr.
      Pages 3-35
  3. Identification of Polyamine Metabolic Enzymes and Characterization of Polyamine-Regulated Genes and Ion Channels

  4. Transgenic Models for Study of Polyamine Function

  5. Enzymes Involved in Polyamine Catabolism and Posttranslational Protein Modification

    1. Front Matter
      Pages 171-171
    2. Andrew C. Goodwin, Tracy R. Murray-Stewart, Robert A. Casero Jr.
      Pages 173-181
    3. Panagiotis N. Moschou, Kalliopi A. Roubelakis-Angelakis
      Pages 183-194
    4. Edith C. Wolff, Seung Bum Lee, Myung Hee Park
      Pages 195-205
    5. Jong Hwan Park, Edith C. Wolff, Myung Hee Park
      Pages 207-216
  6. Regulation of Polyamine Content

    1. Front Matter
      Pages 217-217
    2. Erin K. Willert, Lisa N. Kinch, Margaret A. Phillips
      Pages 219-235

About this book


Recently, important new findings in the polyamine field and a variety of new experimental systems have revolutionized the study of these ubiquitous cellular components, essential for normal growth and development.  In Polyamines: Methods and Protocols, leading researchers contribute an extensive collection of up-to-date laboratory techniques for the further pursuit of polyamine study.  The volume delves into vital subjects such as neoplasia studies with animal models and human patients, therapeutic roles for polyamine inhibitors and analogs, polyamine metabolism and oxidative damage, polyamines as regulators of critical ion channels, as well as polyamine transport systems and polyamine-responsive genes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.


Comprehensive and cutting-edge, Polyamines: Methods and Protocols provides a key resource for all scientists pursuing the study of this dynamic and significant aspect of cellular biology.


Cell growth Ion channel regulation Metabolic enzymes Neoplasia development Oxidative damage Polyamine inhibitors Polyamine metabolism Polyamine-responsive genes Post-translational protein modification Transgenic models Transport systems

Editors and affiliations

  • Anthony E. Pegg
    • 1
  • Robert A. Casero, Jr.
    • 2
  1. 1.College of Medicine, Milton S. Hershey Medical CenterPennsylvania State UniversityHersheyUSA
  2. 2.School of Medicine, Sidney Kimmel ComprehensiveJohns Hopkins UniversityBaltimoreUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-033-1
  • Online ISBN 978-1-61779-034-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book