Methods and Protocols

  • Anthony E. Pegg
  • Robert A. Casero, Jr.

Part of the Methods in Molecular Biology book series (MIMB, volume 720)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Anthony E. Pegg, Robert A. Casero Jr.
      Pages 3-35
  3. Identification of Polyamine Metabolic Enzymes and Characterization of Polyamine-Regulated Genes and Ion Channels

    1. Front Matter
      Pages 37-37
    2. Kazuei Igarashi, Keiko Kashiwagi
      Pages 51-65
    3. Harley T. Kurata, Wayland W. L. Cheng, Colin G. Nichols
      Pages 113-126
  4. Transgenic Models for Study of Polyamine Function

  5. Enzymes Involved in Polyamine Catabolism and Posttranslational Protein Modification

    1. Front Matter
      Pages 171-171
    2. Andrew C. Goodwin, Tracy R. Murray-Stewart, Robert A. Casero Jr.
      Pages 173-181
    3. Panagiotis N. Moschou, Kalliopi A. Roubelakis-Angelakis
      Pages 183-194
    4. Edith C. Wolff, Seung Bum Lee, Myung Hee Park
      Pages 195-205
    5. Jong Hwan Park, Edith C. Wolff, Myung Hee Park
      Pages 207-216
  6. Regulation of Polyamine Content

    1. Front Matter
      Pages 217-217
    2. Erin K. Willert, Lisa N. Kinch, Margaret A. Phillips
      Pages 219-235
    3. Noriyuki Murai, Yasuko Murakami, Senya Matsufuji
      Pages 237-267
    4. Shannon L. Nowotarski, Sofia Origanti, Lisa M. Shantz
      Pages 279-292
  7. Polyamine Transport and Uptake

    1. Front Matter
      Pages 293-293
    2. Marie-Pierre Hasne, Buddy Ullman
      Pages 309-326
    3. Johanna Welch, Katrin Svensson, Paulina Kucharzewska, Mattias Belting
      Pages 327-338
    4. Takeshi Uemura, Eugene W. Gerner
      Pages 339-348
    5. Paul Acheampong, Mary J. Macleod, Heather M. Wallace
      Pages 349-364
  8. Polyamines and Disease

    1. Front Matter
      Pages 365-365
    2. Masao Kawakita, Kyoko Hiramatsu, Mari Yanagiya, Yosuke Doi, Mieko Kosaka
      Pages 367-378
    3. Kazuei Igarashi, Keiko Kashiwagi
      Pages 395-408
    4. Alain P. Gobert, Rupesh Chaturvedi, Keith T. Wilson
      Pages 409-425
    5. Charles E. Schwartz, Xaiojing Wang, Roger E. Stevenson, Anthony E. Pegg
      Pages 437-445
  9. Chemistry and Analysis

    1. Front Matter
      Pages 447-447
    2. Alex R. Khomutov, Janne Weisell, Maxim A. Khomutov, Nikolay A. Grigorenko, Alina R. Simonian, Merja R. Häkkinen et al.
      Pages 449-461
    3. Koichi Takao, Akira Shirahata
      Pages 463-474
    4. Ian S. Blagbrough, Abdelkader A. Metwally, Andrew J. Geall
      Pages 493-503
    5. Merja R. Häkkinen
      Pages 505-518
  10. Back Matter
    Pages 519-523

About this book


Recently, important new findings in the polyamine field and a variety of new experimental systems have revolutionized the study of these ubiquitous cellular components, essential for normal growth and development.  In Polyamines: Methods and Protocols, leading researchers contribute an extensive collection of up-to-date laboratory techniques for the further pursuit of polyamine study.  The volume delves into vital subjects such as neoplasia studies with animal models and human patients, therapeutic roles for polyamine inhibitors and analogs, polyamine metabolism and oxidative damage, polyamines as regulators of critical ion channels, as well as polyamine transport systems and polyamine-responsive genes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.


Comprehensive and cutting-edge, Polyamines: Methods and Protocols provides a key resource for all scientists pursuing the study of this dynamic and significant aspect of cellular biology.


Cell growth Ion channel regulation Metabolic enzymes Neoplasia development Oxidative damage Polyamine inhibitors Polyamine metabolism Polyamine-responsive genes Post-translational protein modification Transgenic models Transport systems

Editors and affiliations

  • Anthony E. Pegg
    • 1
  • Robert A. Casero, Jr.
    • 2
  1. 1.College of Medicine, Milton S. Hershey Medical CenterPennsylvania State UniversityHersheyUSA
  2. 2.School of Medicine, Sidney Kimmel ComprehensiveJohns Hopkins UniversityBaltimoreUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-033-1
  • Online ISBN 978-1-61779-034-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site