Small Molecule Microarrays

Methods and Protocols

  • Mahesh Uttamchandani
  • Shao Q. Yao

Part of the Methods in Molecular Biology book series (MIMB, volume 669)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Mahesh Uttamchandani, Shao Q. Yao
    Pages 1-15
  3. Dhaval Gosalia, Scott L. Diamond
    Pages 69-78
  4. Kang L. D. Aw, Shao Q. Yao, Mahesh Uttamchandani
    Pages 79-93
  5. Isao Miyazaki, Siro Simizu, Hiroyuki Osada
    Pages 95-107
  6. Alexander Nesterov, Edgar Dörsam, Yun-Chien Cheng, Christopher Schirwitz, Frieder Märkle, Felix Löffler et al.
    Pages 109-124
  7. Gaëlle Piret, Hervé Drobecq, Rabah Boukherroub, Oleg Melnyk
    Pages 125-133
  8. Rémi Desmet, Eric Diesis, Hervé Drobecq, Carine Rouanet, Karim Chemlal, Anne-Sophie Debrie et al.
    Pages 135-145
  9. Marina Cretich, Francesco Damin, Renato Longhi, Cecilia Gotti, Clelia Galati, Lucio Renna et al.
    Pages 147-160
  10. Antonia Masch, Johannes Zerweck, Ulf Reimer, Holger Wenschuh, Mike Schutkowski
    Pages 161-172
  11. Alexandra Thiele, Matthias Weiwad, Johannes Zerweck, Gunter Fischer, Mike Schutkowski
    Pages 173-181
  12. Olga Aminova, Matthew D. Disney
    Pages 209-224
  13. Back Matter
    Pages 225-228

About this book

Introduction

Small molecule microarrays (SMM) were introduced just a decade ago in 1999 and, within a short space of time, have already established themselves as a vibrant, next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols showcases a collection of contributions guiding researchers toward ways in which small molecule microarray technology may be deployed for multiplexed screening and profiling. Organized by the categories of small molecules presented on the microarrays, this detailed volume describes in-depth techniques for chemical libraries, peptide libraries, and carbohydrate microarrays. Written in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, vital tips on troubleshooting this often difficult technology, and advice on avoiding known pitfalls. Authoritative and cutting-edge, Small Molecule Microarrays: Methods and Protocols provides meticulous depictions of key hands-on experience and seeks to inspire a future generation of microarray practitioners to take this significant technology forward.

Keywords

Array fabrication Carbohydrates Chemical and peptide libraries Enzyme profiling Hydra Inhibitor discovery Library synthesis Lysate protein screening Microarray Molecular immobilization SMM enzymes molecular biology

Editors and affiliations

  • Mahesh Uttamchandani
    • 1
  • Shao Q. Yao
    • 2
  1. 1., DSO National LaboratoriesDefence Medical and Environmental ResearSingaporeSingapore
  2. 2.Faculty of Science, Depts. Chemistry & Biological SciencesNational University of SingaporeSingaporeSingapore

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-845-4
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-844-7
  • Online ISBN 978-1-60761-845-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book