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MicroRNAs and the Immune System

Methods and Protocols

  • Book
  • © 2010


  • Offers cutting edge methods to analyse microRNA expression, function and post-transcriptional modifications
  • Includes methods to isolate and manipulate cells of the immune system
  • Provides a description of bioinformatics databases on miRNAs and their targets, as well as instructions to navigate them
  • Includes supplementary material:

Part of the book series: Methods in Molecular Biology (MIMB, volume 667)

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About this book

In recent years, the critical role of microRNAs has been revealed within the biology of cells that constitute the immune system. In MicroRNAs and the Immune System: Methods and Protocols, expert researchers explore the latest techniques for studying miRNA expression, including the most up-to-date data on splinted ligation and qRT-PCR assays, as well as high-throughput profiling through cloning, deep sequencing, and microarrays. Chapters outline methods to study miRNA functions in various cell types from a single cell type level to entire model organisms, and present studies of miRNAs in the context of viruses and the immune response. Tools are also provided to help navigate bioinformatics databases on miRNAs and their targets. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Contemporary and innovative, MicroRNAs and the Immune System: Methods and Protocols is an essential handbook for immunologists, biochemists, and molecular biologists.

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Table of contents (21 protocols)

  1. Analysis of miRNA Expression: Classic Methods Revisited

  2. High-Throughput Analysis of miRNAs

  3. Functional Analysis of miRNAs in the Immune System: Gain-of-Function

  4. Functional Analysis of miRNAs in the Immune System: Loss-of-Function


From the reviews:

“This book is useful to scientists seeking to study the role of miRNAs in the immune system. … helpful to immunologists, biochemists, and molecular biologists interested in miRNA effects. The detailed protocols are invaluable to students and post-docs in particular. … The figures of the types of data one may obtain and the notes on troubleshooting are invaluable to both seasoned investigators and novice students.” (Elizabeth S. Raveche, Doody’s Review Service, February, 2011)

“Serves primarily as a recipe book, detailing all the latest methods one would need to further investigate the roles of microRNAs in this area of biology. It will appeal to both students who are new to the field … and molecular biologists who can keep it close as a handy reference material for planning and carrying out their experiments. … appealing to anyone from beginners to experts. … an attractive book for those interested in identifying and characterising particular miRNAs in the immune system.” (Daniel Peters, Immunology News, February, 2011)

Editors and Affiliations

  • Institute for Research in Biomedicine, Bellinzona, Switzerland

    Silvia Monticelli

Bibliographic Information

  • Book Title: MicroRNAs and the Immune System

  • Book Subtitle: Methods and Protocols

  • Editors: Silvia Monticelli

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science + Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-810-2Published: 13 September 2010

  • Softcover ISBN: 978-1-4939-6150-4Published: 23 August 2016

  • eBook ISBN: 978-1-60761-811-9Published: 09 September 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 330

  • Topics: Immunology

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