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Fluorescence in situ Hybridization (FISH)

Protocols and Applications

  • Book
  • © 2010

Overview

Editors:
  1. Joanna M. Bridger

    1. School of Health Sciences & Social Care, Div. Biosciences, Brunel University, Uxbridge, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  2. Emanuela V. Volpi

    1. , Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Contains a plethora of modern adaptations to the FISH methodology
  • Of great use to both clinical and research laboratories
  • Will inform how to achieve and recognize quality in the different FISH methodologies
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 659)

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Table of contents (34 protocols)

  1. Translational FISH: Applications for Human Genetics and Medicine

    1. Front Matter

      Pages 247-247
      Download chapter PDF

    2. Quality Control in FISH as Part of a Laboratory’s Quality Management System

      • Ros Hastings
      Pages 249-259

    3. FlashFISH: “Same Day” Prenatal Diagnosis of Common Chromosomal Aneuploidies

      • Sherry S. Y. Ho, Mahesh A. Choolani
      Pages 261-268

    4. FISH for Pre-implantation Genetic Diagnosis

      • Paul N. Scriven, Caroline Mackie Ogilvie
      Pages 269-282

    5. PNA–FISH on Human Sperm

      • Franck Pellestor, Cécile Monzo, Samir Hamamah
      Pages 283-289

    6. POD-FISH: A New Technique for Parental Origin Determination Based on Copy Number Variation Polymorphism

      • Anja Weise, Madeleine Gross, Sophie Hinreiner, Vera Witthuhn, Hasmik Mkrtchyan, Thomas Liehr
      Pages 291-298

    7. Sequence-Based High Resolution Chromosomal Comparative Genomic Hybridization (CGH)

      • Agata Kowalska, Eva Bozsaky, Peter F. Ambros
      Pages 299-312

    8. ImmunoFISH on Isolated Nuclei from Paraffin-Embedded Biopsy Material

      • Soo-Yong Tan, Goran Mattsson
      Pages 313-321

    9. Fluorescence In Situ Hybridization on 3D Cultures of Tumor Cells

      • Karen J. Meaburn
      Pages 323-336

    10. Simultaneous Ultrasensitive Subpopulation Staining/Hybridization In Situ (SUSHI) in HIV-1 Disease Monitoring

      • Bruce K. Patterson
      Pages 337-346

  2. Protocols for Model Organisms

    1. Front Matter

      Pages 347-347
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    2. Detection of Prokaryotic Cells with Fluorescence In Situ Hybridization

      • Katrin Zwirglmaier
      Pages 349-362

    3. FISH as a Tool to Investigate Chromosome Behavior in Budding Yeast

      • Harry Scherthan, Josef Loidl
      Pages 363-377

    4. FISH on Chromosomes Derived from the Snail Model Organism Biomphalaria glabrata

      • Edwin C. Odoemelam, Nithya Raghavan, Wannaporn Ittiprasert, Andre Miller, Joanna M. Bridger, Matty Knight
      Pages 379-388

    5. Fluorescence in situ Hybridization with Bacterial Artificial Chromosomes (BACs) to Mitotic Heterochromatin of Drosophila

      • Maria Carmela Accardo, Patrizio Dimitri
      Pages 389-400

    6. Three-Dimensional Fluorescence In Situ Hybridization in Mouse Embryos Using Repetitive Probe Sequences

      • Walid E. Maalouf, Tiphaine Aguirre-Lavin, Laetitia Herzog, Isabelle Bataillon, Pascale Debey, Nathalie Beaujean
      Pages 401-408

    7. Fluorescence in situ Hybridization (FISH) for Genomic Investigations in Rat

      • Andrew Jefferson, Emanuela V. Volpi
      Pages 409-426

    8. Fluorescence In Situ Hybridization on Early Porcine Embryos

      • Helen A. Foster, Roger G. Sturmey, Paula J. Stokes, Henry J. Leese, Joanna M. Bridger, Darren K. Griffin
      Pages 427-436

    9. FISH on 3D Preserved Bovine and Murine Preimplantation Embryos

      • Daniela Koehler, Valeri Zakhartchenko, Nina Ketterl, Eckhard Wolf, Thomas Cremer, Alessandro Brero
      Pages 437-445

  3. Back Matter

    Pages 447-451
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Keywords

About this book

Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics. The design simplicity and cost-effectiveness of the early FISH protocols, combined with the signifcant acceleration of discoveries in related technical areas such as fuor- cence microscopy, digital imaging, and nucleic acid technology have prompted the div- sifcation of the original technique into an outstanding number of imaginative and useful applications, and thus have not only held back its outmoding but have also promoted its expansion into different areas of basic and applied research in the post-genomic era. The 34 chapters included in this book aim at portraying the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of va- ous applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology, and evolutionary st- ies. The book is divided in four parts: (I) Core Techniques, (II) Technical Advancements and Novel Adaptations, (III) Translational FISH: Applications for Human Genetics and Medicine, and (IV) Protocols for Model Organisms.

Reviews

From the reviews:

“Fluorescence in situ hybridization (FISH) has been widely adopted to enable the study of uncultured target cells. This book shows many more applications in cytology. Relatively few chapters are devoted directly to microbiological applications; the strong theme of the book describes methods targeting chromosomes. … Protocols on 3-D FISH may have useful material for microbiologists, e.g. studies on biofilms. … I would suggest this is for microbiological libraries … .” (Jonathan Porter, Microbiology Today, November, 2011)

Editors and Affiliations

  • School of Health Sciences & Social Care, Div. Biosciences, Brunel University, Uxbridge, United Kingdom

    Joanna M. Bridger

  • , Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom

    Emanuela V. Volpi

Bibliographic Information

  • Book Title: Fluorescence in situ Hybridization (FISH)

  • Book Subtitle: Protocols and Applications

  • Editors: Joanna M. Bridger, Emanuela V. Volpi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-789-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-788-4Published: 09 September 2010

  • Softcover ISBN: 978-1-4939-5724-8Published: 23 August 2016

  • eBook ISBN: 978-1-60761-789-1Published: 02 September 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 451

  • Topics: Biochemistry, general, Animal Genetics and Genomics, Human Genetics, Cytogenetics

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