Fluorescence in situ Hybridization (FISH)

Protocols and Applications

  • Joanna M. Bridger
  • Emanuela V. Volpi

Part of the Methods in Molecular Biology book series (MIMB, volume 659)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Core Techniques

  3. Technical Advancements and Novel Adaptations

    1. Front Matter
      Pages 127-127
    2. Asli N. Silahtaroglu
      Pages 165-171
    3. Susan M. Bailey, Eli S. Williams, Michael N. Cornforth, Edwin H. Goodwin
      Pages 173-183
    4. Eberhard Schmitt, Jutta Schwarz-Finsterle, Stefan Stein, Carmen Boxler, Patrick Müller, Andriy Mokhir et al.
      Pages 185-202
    5. Sheila Q. Xie, Liron-Mark Lavitas, Ana Pombo
      Pages 219-230
    6. Thomas Liehr, Anja Weise, Sophie Hinreiner, Hasmik Mkrtchyan, Kristin Mrasek, Nadezda Kosyakova
      Pages 231-238
    7. Joop Wiegant, Anneke K. Brouwer, Hans J. Tanke, Roeland W. Dirks
      Pages 239-246
  4. Translational FISH: Applications for Human Genetics and Medicine

    1. Front Matter
      Pages 247-247
    2. Paul N. Scriven, Caroline Mackie Ogilvie
      Pages 269-282
    3. Franck Pellestor, Cécile Monzo, Samir Hamamah
      Pages 283-289
    4. Anja Weise, Madeleine Gross, Sophie Hinreiner, Vera Witthuhn, Hasmik Mkrtchyan, Thomas Liehr
      Pages 291-298
    5. Agata Kowalska, Eva Bozsaky, Peter F. Ambros
      Pages 299-312
  5. Protocols for Model Organisms

    1. Front Matter
      Pages 347-347
    2. Harry Scherthan, Josef Loidl
      Pages 363-377
    3. Edwin C. Odoemelam, Nithya Raghavan, Wannaporn Ittiprasert, Andre Miller, Joanna M. Bridger, Matty Knight
      Pages 379-388
    4. Walid E. Maalouf, Tiphaine Aguirre-Lavin, Laetitia Herzog, Isabelle Bataillon, Pascale Debey, Nathalie Beaujean
      Pages 401-408
    5. Andrew Jefferson, Emanuela V. Volpi
      Pages 409-426
    6. Helen A. Foster, Roger G. Sturmey, Paula J. Stokes, Henry J. Leese, Joanna M. Bridger, Darren K. Griffin
      Pages 427-436
    7. Daniela Koehler, Valeri Zakhartchenko, Nina Ketterl, Eckhard Wolf, Thomas Cremer, Alessandro Brero
      Pages 437-445
  6. Back Matter
    Pages 447-451

About this book


The design simplicity and cost-effectiveness of the early Fluorescence in situ Hybridization (FISH) protocols, combined with the significant acceleration of discoveries in related technical areas like fluorescence microscopy, digital imaging, and nucleic acid technology have prompted the diversification of the original technique into an outstanding number of imaginative and useful applications, thus promoting its expansion into different areas of basic and applied research in the post-genomic era. In Fluorescence in situ Hybridization (FISH): Protocols and Applications, experts in the field portray the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of various applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology and evolutionary studies. The book is divided into four convenient sections covering the core techniques, technical advancements and novel adaptations, applications for human genetics and medicine, as well as protocols for model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Fluorescence in situ Hybridization (FISH): Protocols and Applications aims to aid scientists from across the world in applying this tried-and-true scientific technique to their own lab’s current research.


Chromosom DNA DNA halos FISH Fluorescence microscopy Fluoreszenz in situ-Hybridisierung Genetic-based diagnostics & prognosis Microarray Model organisms Molecular cytogenetics Translational FISH genetics hybridization molecular biology tissue

Editors and affiliations

  • Joanna M. Bridger
    • 1
  • Emanuela V. Volpi
    • 2
  1. 1.School of Health Sciences & Social Care, Div. BiosciencesBrunel UniversityUxbridgeUnited Kingdom
  2. 2., Wellcome Trust Centre for Human GeneticsUniversity of OxfordOxfordUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-788-4
  • Online ISBN 978-1-60761-789-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site