Fluorescence in situ Hybridization (FISH)

Protocols and Applications

  • Joanna M. Bridger
  • Emanuela V. Volpi

Part of the Methods in Molecular Biology book series (MIMB, volume 659)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Core Techniques

  3. Technical Advancements and Novel Adaptations

    1. Front Matter
      Pages 127-127
    2. Asli N. Silahtaroglu
      Pages 165-171
    3. Susan M. Bailey, Eli S. Williams, Michael N. Cornforth, Edwin H. Goodwin
      Pages 173-183
    4. Eberhard Schmitt, Jutta Schwarz-Finsterle, Stefan Stein, Carmen Boxler, Patrick Müller, Andriy Mokhir et al.
      Pages 185-202
    5. Sheila Q. Xie, Liron-Mark Lavitas, Ana Pombo
      Pages 219-230
    6. Thomas Liehr, Anja Weise, Sophie Hinreiner, Hasmik Mkrtchyan, Kristin Mrasek, Nadezda Kosyakova
      Pages 231-238
    7. Joop Wiegant, Anneke K. Brouwer, Hans J. Tanke, Roeland W. Dirks
      Pages 239-246

About this book

Introduction

The design simplicity and cost-effectiveness of the early Fluorescence in situ Hybridization (FISH) protocols, combined with the significant acceleration of discoveries in related technical areas like fluorescence microscopy, digital imaging, and nucleic acid technology have prompted the diversification of the original technique into an outstanding number of imaginative and useful applications, thus promoting its expansion into different areas of basic and applied research in the post-genomic era. In Fluorescence in situ Hybridization (FISH): Protocols and Applications, experts in the field portray the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of various applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology and evolutionary studies. The book is divided into four convenient sections covering the core techniques, technical advancements and novel adaptations, applications for human genetics and medicine, as well as protocols for model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Fluorescence in situ Hybridization (FISH): Protocols and Applications aims to aid scientists from across the world in applying this tried-and-true scientific technique to their own lab’s current research.

Keywords

Chromosom DNA DNA halos FISH Fluorescence microscopy Fluoreszenz in situ-Hybridisierung Genetic-based diagnostics & prognosis Microarray Model organisms Molecular cytogenetics Translational FISH genetics hybridization molecular biology tissue

Editors and affiliations

  • Joanna M. Bridger
    • 1
  • Emanuela V. Volpi
    • 2
  1. 1.School of Health Sciences & Social Care, Div. BiosciencesBrunel UniversityUxbridgeUnited Kingdom
  2. 2., Wellcome Trust Centre for Human GeneticsUniversity of OxfordOxfordUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-789-1
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-788-4
  • Online ISBN 978-1-60761-789-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book