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RNAi and microRNA-Mediated Gene Regulation in Stem Cells

Methods, Protocols, and Applications

  • Baohong Zhang
  • Edmund J. Stellwag

Part of the Methods in Molecular Biology book series (MIMB, volume 650)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introduction

    1. Front Matter
      Pages 1-1
  3. RNA Interference

    1. Front Matter
      Pages 27-27
    2. Christine Karlsson, Jonas Larsson, Aurélie Baudet
      Pages 29-43
    3. Soizik Berlivet, Martin Houlard, Matthieu Gérard
      Pages 85-100
    4. Carsten Hagemann, Harun M. Said, Michael Flentje, Klaus Roosen, Giles Hamilton Vince
      Pages 129-143
    5. Jun-Yang Liou, Bor-Sheng Ko, Tzu-Ching Chang
      Pages 145-153
  4. MicroRNAs

    1. Front Matter
      Pages 171-171
    2. Ryan D. Morin, Yongjun Zhao, Anna-Liisa Prabhu, Noreen Dhalla, Helen McDonald, Pawan Pandoh et al.
      Pages 173-199
    3. Xiaoping Pan, Alexander K. Murashov, Edmund J. Stellwag, Baohong Zhang
      Pages 213-224
  5. Back Matter
    Pages 257-261

About this book

Introduction

Recent stem cell research has revealed that miRNA and RNAi-mediated gene regulation is one of the vital determinates controlling the state of cell differentiation, with the small RNAs serving as key elements involved in regulatory network control of pluripotent cell fate determination. In RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications, expert authors from laboratories across the globe contribute an accessible compendium of up-to-date, proven methods focused on the study of the titular topic. Divided into three sections, the book first gives a brief introduction to RNAi and miRNAs in stem cells, with a focus on the current status of research and future perspectives, then it continues with detailed methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, concluding with a section on recently developed methods for identification of miRNAs, including a general protocol for preparation and analysis of miRNA libraries for deep sequencing, knock down of a specific gene using miRNA-based shRNA, and miRNA expression analysis using qRT-PCR. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications serves as a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and the exciting areas of medicine, including regenerative medicine, aging, cancer, and neurological disorders, that can be advanced through this expanding area of research.

Keywords

Migration PCR Termination Vivo gene expression genes miRNAs molecular biology regulation

Editors and affiliations

  • Baohong Zhang
    • 1
  • Edmund J. Stellwag
    • 2
  1. 1.Dept. BiologyEast Carolina UniversityGreenvilleUSA
  2. 2.Dept. BiologyEast Carolina UniversityGreenvilleUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-769-3
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-768-6
  • Online ISBN 978-1-60761-769-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site