Overview
- Provides an easily accessible reference volume for plant stress tolerance written by leading researchers in the field
- Details the wide range of assays relevant to assessing plant stress tolerance
- Provides detailed step-by-step instructions for the protocols covering a whole range of topics in plant stress tolerance
- Details the high-throughput identification of stress tolerance genes, proteins, and microRNAs
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 639)
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About this book
A number of abiotic factors such as drought, salinity, extreme temperatures, low or high light intensity, and deficiency or toxic levels of nutrients have huge impacts on crop productivity, and a furthering of our understanding of the molecular, biochemical, and physiological basis of stress tolerance has been widely recognized as critical. In Plant Stress Tolerance: Methods and Protocols, expert researchers cover the most important widely-used techniques, including cutting-edge strategies, in a manner that ensures effective results. Beginning with reviews on dehydration, salinity, and cold tolerance as well as on oxidative stress, the volume then continues with methods involving topics such as describing the identification of stress-regulated genes, proteins, and microRNAs using diverse approaches, measurement of osmotic adjustment, proline levels, enzymes involved in proline metabolism, and sugars as well as determination of ROS levels, lipid peroxidation, ion leakage, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Plant Stress Tolerance: Methods and Protocols provides a wide range of easy-to-follow protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.
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Keywords
Table of contents (23 protocols)
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Front Matter
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Reviews and Overview Chapters
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Front Matter
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Genome-Wide Approaches for Identification of Stress-Regulated Genes, Proteins, and Small RNAs
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Front Matter
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Genome-Wide Approaches for Identification of Stress Regulated Genes, Proteins, and Small RNAs
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Antioxidant Enzymes and Metabolites
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Front Matter
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Reviews
From the reviews:
“The present works include 4 thematic areas that are developed along 23 chapters which focus, among others, basic aspects of the oxidative stress, the cold tolerance, the salinity and the analysis of transcriptomes related to these stress. … this work constitutes a valuable contribution to the researchers involved in the study of abiotic stress that affect the crops.” (Pascual Franzone, Integrated Pest Management Bulletin, December, 2010)Editors and Affiliations
Bibliographic Information
Book Title: Plant Stress Tolerance
Book Subtitle: Methods and Protocols
Editors: Ramanjulu Sunkar
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-702-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2010
Hardcover ISBN: 978-1-60761-701-3Published: 14 May 2010
Softcover ISBN: 978-1-4939-5741-5Published: 23 August 2016
eBook ISBN: 978-1-60761-702-0Published: 13 April 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 386
Topics: Plant Physiology, Plant Sciences