Plant Epigenetics

Methods and Protocols

  • Igor Kovalchuk
  • Franz J. Zemp

Part of the Methods in Molecular Biology™ book series (MIMB, volume 631)

Table of contents

  1. Front Matter
    Pages i-x
  2. Andrea M. Foerster, Ortrun Mittelsten Scheid
    Pages 1-11
  3. Andrea M. Foerster, Jennifer Hetzl, Christoph Müllner, Ortrun Mittelsten Scheid
    Pages 13-22
  4. Palak Kathiria, Igor Kovalchuk
    Pages 41-48
  5. Leonor Ruiz-García, Jose Antonio Cabezas, Nuria de María, María-Teresa Cervera
    Pages 63-74
  6. Martine Boccara, Alexis Sarazin, Bernard Billoud, Agnes Bulski, Louise Chapell, David Baulcombe et al.
    Pages 75-86
  7. Erika Varkonyi-Gasic, Roger P. Hellens
    Pages 109-122
  8. Yuko Tagami, Naoko Inaba, Yuichiro Watanabe
    Pages 123-138
  9. Julia Engelhorn, Franziska Turck
    Pages 185-207
  10. Andrea T. Todd, Enwu Liu, Jonathan E. Page
    Pages 221-236
  11. Alicja Ziemienowicz
    Pages 253-268
  12. Back Matter
    Pages 269-273

About this book


The past fifteen years have witnessed major advances in epigenetics, one of the most popular and quickly evolving fields of modern science. In Plant Epigenetics: Methods and Protocols, expert researchers explore the most recent developments, examining in great detail the contribution of epigenetic regulation to cell function in plants. Chapters include a variety of protocols for studying the function of small non-coding RNAs, DNA methylation, and histone modifications in plants, often in different degrees of complexity. This volume describes bioinformatic approaches to the analysis of high-throughput data, such as bisulfite sequencing and Chip-on-chip assays. It features much-desired protocols for plant transgenesis and the analysis of genome stability, with a detailed discussion of their applications to epigenetic studies. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Wide-ranging and innovative, Plant Epigenetics: Methods and Protocols is an invaluable manual designed to help researchers uncover the undiscovered and unexplained phenomena in plant biology.


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Editors and affiliations

  • Igor Kovalchuk
    • 1
  • Franz J. Zemp
    • 2
  1. 1.Dept. Biological SciencesUniversity of LethbridgeLethbridgeCanada
  2. 2.Dept. Biological SciencesUniversity of LethbridgeLethbridgeCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-645-0
  • Online ISBN 978-1-60761-646-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site