Overview
- Describes all most recent advances in lentivirus vector technology
- Introduces lentiviral vectors as therapeutic tools for nervous and inherited disorders
- Illustrates new ways to regulate cell differentiation and elicit potent immune responses
- Presents new and simple methods to create transgenic animals
- Details the application of microRNA technology by lentiviral vectors
Part of the book series: Methods in Molecular Biology (MIMB, volume 614)
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About this book
Since the publication of the first edition, lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, continue to offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. Lentivirus Gene Engineering Protocols, Second Edition reflects the spectacular progress made in the field with a set of cutting-edge methods contributed by highly respected scientists. Beginning with a thorough overview of the most recent lentivirus developments, the book continues with detailed protocols including sections on the advances in lentiviral vector technology, new lentiviral vector applications, involving transgenic human embryonic stem cells and fetal gene therapy among other topics, as well as the invaluable breakthroughs in LV-mediated expression of microRNAs. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
Authoritative and timely, Lentivirus Gene Engineering Protocols, Second Edition covers the most relevant issues and techniques of LV-based gene engineering, thus representing a complete theoretical and practical guide for scientists still unfamiliar with LV technologies and those who simply wish to know more about this vital area of study.
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Keywords
Table of contents (15 protocols)
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Front Matter
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Advances in Lentiviral Vector Technology
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Front Matter
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New Lentiviral Vector Applications
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Front Matter
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The Short RNA Technologies Applied by Lentiviral Vectors
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Front Matter
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Editors and Affiliations
Bibliographic Information
Book Title: Lentivirus Gene Engineering Protocols
Editors: Maurizio Federico
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-533-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2010
Hardcover ISBN: 978-1-60761-532-3Published: 21 December 2009
Softcover ISBN: 978-1-4939-5653-1Published: 23 August 2016
eBook ISBN: 978-1-60761-533-0Published: 12 March 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XII, 228
Additional Information: Originally published as volume 229 in the series: Methods Molecular Biology
Topics: Genetic Engineering, Biochemistry, general, Cell Biology