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Live Cell Imaging

Methods and Protocols

  • Dmitri B. Papkovsky

Part of the Methods in Molecular Biology book series (MIMB, volume 591)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Part I: General Principles and Overview

  3. Part II: Imaging Techniques, Probes, and Applications

    1. Front Matter
      Pages 67-67
    2. Elizabeth Pham, Kevin Truong
      Pages 69-91
    3. Dan Yang, Zhen-Ning Sun, Tao Peng, Hua-Li Wang, Jian-Gang Shen, Yan Chen et al.
      Pages 93-103
    4. Christian A. Wurm, Daniel Neumann, Roman Schmidt, Alexander Egner, Stefan Jakobs
      Pages 185-199
    5. Oleg Gerasimenko, Julia Gerasimenko
      Pages 201-210
    6. Benedikt W. Graf, Stephen A. Boppart
      Pages 211-227
    7. Gilles Charvin, Catherine Oikonomou, Frederick Cross
      Pages 229-242
    8. Joachim Fandrey, Christoph Wotzlaw
      Pages 243-255
    9. Andreas Fercher, Tomas C. O’Riordan, Alexander V. Zhdanov, Ruslan I. Dmitriev, Dmitri B. Papkovsky
      Pages 257-273
    10. Martin vandeVen, Corina Balut, Szilvia Baron, Ilse Smets, Paul Steels, Marcel Ameloot
      Pages 275-309
    11. Daniel C. Worth, Maddy Parsons
      Pages 311-323
    12. Manus W. Ward
      Pages 335-351
    13. Jeremy Bonor, Anja Nohe
      Pages 353-364
  4. Back Matter
    Pages 365-367

About this book

Introduction

Now a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable.  In Live Cell Imaging: Methods and Protocols, a panel of expert contributors provide a comprehensive compendium of experimental approaches to live cell imaging in the form of several overview chapters followed by representative examples and case studies covering different aspects of the most current methodology. By examining a range of state-of-the-art protocols extensively validated in complex biological studies, this volume highlights new experimental and instrumental opportunities and helps researchers to select appropriate imaging methods for their specific biological questions and measurement tasks.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Live Cell Imaging: Methods and Protocols promises to contribute greatly to the further development and dissemination of this fundamentally important technology which spans across many disciplines including molecular and cell biology, chemistry, physics, optics, engineering, cell physiology, and medicine.

Keywords

Data analysis algorithms Multi-mode microscopes Multi-photon excitation Optoelectronics Probes Sample manipulation

Editors and affiliations

  • Dmitri B. Papkovsky
    • 1
  1. 1.Dept. BiochemistryUniversity College CorkCorkIreland

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-404-3
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-403-6
  • Online ISBN 978-1-60761-404-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site