Human Embryonic Stem Cell Protocols

  • Kursad Turksen

Part of the Methods in Molecular Biology book series (MIMB, volume 584)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Teija T. Peura, Julia Schaft, Tomas Stojanov
    Pages 21-54
  3. Carmen Escobedo-Lucea, Miodrag Stojkovic
    Pages 55-69
  4. John A. Ozolek, Esther P. Jane, James E. Esplen, Patti Petrosko, Amy K. Wehn, Teresa M. Erb et al.
    Pages 71-95
  5. Isabelle Peiffer, Romain Barbet, Antoinette Hatzfeld, Ma-Lin Li, Jacques A. Hatzfeld
    Pages 97-108
  6. Katherine E. Wagner, Mohan C. Vemuri
    Pages 109-119
  7. Amparo Galán, Carlos Simón
    Pages 135-150
  8. Christian B. Brøchner, Peter S. Vestentoft, Niels Lynnerup, Claus Yding Andersen, Kjeld Møllgård
    Pages 179-193
  9. Aashir Awan, Roberto S. Oliveri, Pernille L. Jensen, Søren T. Christensen, Claus Yding Andersen
    Pages 195-210
  10. Raymond C.B. Wong, Alice Pébay
    Pages 211-228
  11. Ying Liu, Uma Lakshmipathy, Ali Ozgenc, Bhaskar Thyagarajan, Pauline Lieu, Andrew Fontes et al.
    Pages 229-268
  12. Y. Murat Elçin, Bülend İnanç, A. Eser Elçin
    Pages 269-281
  13. Ronald S. Goldstein, Oz Pomp, Irina Brokhman, Lina Ziegler
    Pages 283-300
  14. Susan E. Zabierowski, Meenhard Herlyn
    Pages 301-316
  15. Wei Seong Toh, Eng Hin Lee, Mark Richards, Tong Cao
    Pages 317-331
  16. Sharon Gerecht*, Lino S. Ferreira*, Robert Langer
    Pages 333-354

About this book

Introduction

Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, new freedoms in regard to using them for research has allowed interest to remain high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, Second Edition, internationally respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. As a volume of the Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols, Second Edition offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today.

Keywords

Cell culture HES cells Lineage commitment PCR Proteomics Regenerative medicine Self-renewal cell lines human embryonic stem cell lines molecular biology

Editors and affiliations

  • Kursad Turksen
    • 1
  1. 1.Sprott Centre for Stem Cell ResearchOttawa Hosp. Research Institute (OHRI)OttawaCanada

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-369-5
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-368-8
  • Online ISBN 978-1-60761-369-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book