Natural Killer Cell Protocols

Cellular and Molecular Methods

  • Kerry S. Campbell

Part of the Methods in Molecular Biology book series (MIMB, volume 612)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Yoshihiro Hayakawa, Daniel M. Andrews, Mark J. Smyth
    Pages 27-38
  3. A. Helena Jonsson, Wayne M. Yokoyama
    Pages 39-49
  4. Michael E. March, Catharina C. Gross, Eric O. Long
    Pages 67-88
  5. Deborah N. Burshtyn, Chelsea Davidson
    Pages 89-96
  6. Mathias Lucas, Cedric Vonarbourg, Peter Aichele, Andreas Diefenbach
    Pages 97-126
  7. Keri B. Sanborn, Gregory D. Rak, Ashley N. Mentlik, Pinaki P. Banerjee, Jordan S. Orange
    Pages 127-148
  8. Alexander W. MacFarlane IV, James F. Oesterling, Kerry S. Campbell
    Pages 149-157
  9. Lukasz K. Chlewicki, Vinay Kumar
    Pages 177-198
  10. S. M. Shahjahan Miah, Kerry S. Campbell
    Pages 199-208
  11. Ram Savan, Tim Chan, Howard A. Young
    Pages 209-221
  12. Amanda K. Purdy, Kerry S. Campbell
    Pages 223-231
  13. Dikla Lankry, Roi Gazit, Ofer Mandelboim
    Pages 249-273
  14. Alon Zilka, Michal Mendelson, Benyamin Rosental, Oren Hershkovitz, Angel Porgador
    Pages 275-283
  15. Yoshie-Matsubayashi Iizuka, Nikunj V. Somia, Koho Iizuka
    Pages 285-297

About this book

Introduction

As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation. While the first edition provided a valuable collection of classical cellular and in vivo techniques to study NK cell functions, the Second Edition of Natural Killer Cell Protocols: Cellular and Molecular Methods brings together more recently developed methods, more refined techniques, and detailed protocols designed to study NK cells within specialized tissue sites in both mice and humans. In this collection of methods, international leaders in the field cover topics ranging from the analysis of the various stages of NK cell development and maturation to specialized techniques for the identification of ligands for NK cell receptors. This volume also includes an appendix, providing a rich resource summarizing available reagents to study NK cells, cross-referencing KIR nomenclature, and detailing the many HLA ligands for various KIR family members. As a volume in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and thorough notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Natural Killer Cell Protocols: Cellular and Molecular Methods, Second Edition seeks to aid researchers and further advance our understanding of the functions, maturation, and regulation of these fascinating and dynamic cells.

Keywords

Activation CMV Malaria PCR Promoter Transplantation Vivo cell lines genes inbred mouse strains infections molecular biology red blood cells regulation

Editors and affiliations

  • Kerry S. Campbell
    • 1
  1. 1.Institute for Cancer ResearchFox Chase Cancer CenterPhiladelphiaU.S.A.

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-362-6
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-361-9
  • Online ISBN 978-1-60761-362-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book