Lipidomics

Volume 1: Methods and Protocols

  • Donald Armstrong

Part of the Methods in Molecular Biology book series (MIMB, volume 579)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Shotgun and Global Lipidomics

  3. Analytical Approaches

    1. Front Matter
      Pages 88-88
    2. Engy A. Mahrous, Robin B. Lee, Richard E. Lee
      Pages 89-102
    3. Beate Fuchs, Ariane Nimptsch, Rosmarie Süß, Jürgen Schiller
      Pages 103-125
    4. Peter J. Quinn, D. Rainteau, C. Wolf
      Pages 127-159
    5. Susanna L. Lundström, Fabio L. D’Alexandri, Kasem Nithipatikom, Jesper Z. Haeggström, Åsa M. Wheelock, Craig E. Wheelock
      Pages 161-187
    6. Giuseppe Astarita, Faizy Ahmed, Daniele Piomelli
      Pages 201-219
    7. Timothy J. Garrett, William W. Dawson
      Pages 247-260
    8. Wooki Kim, Robert S. Chapkin, Rola Barhoumi, David W. L. Ma
      Pages 261-270
    9. Hiroki Nakanishi, Hideo Ogiso, Ryo Taguchi
      Pages 287-313
    10. Etienne Pouteau, Carine Beysen, Nabil Saad, Scott Turner
      Pages 337-358

About this book

Introduction

Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols, an international panel of experts present a wide variety of reviewed as well as unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics. These studies range from simple systems, such as in yeast, to complex biological models. Samples used in these studies include: mitochondria, microsomes, endosomal membranes, RBS and plasma membranes, vesicles, caveolae, liposomes, macrophages, meibum, brain, microglia, retina, adipose tissue, keratinocytes, pulmonary surfactant, and hepatic cultures.  Volume 1 focuses on shotgun and global lipidomics, analytical approaches, and lipid maps. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include useful introductions to their respective topics, lists of the necessary equipment and materials, step-by-step, readily reproducible laboratory protocols, and authoritative notes on troubleshooting and avoiding common pitfalls.

Comprehensive and cutting-edge, Lipidomics: Methods and Protocols will support researchers who wish to increase their utilization of lipidomics, which will certainly lead to more powerful technology, improved diagnostic-prognostic capabilities, and the identification of new valuable products.

Keywords

Macrophages Proteomics Termination bioinformatics biological functions liposomes molecular biology

Editors and affiliations

  • Donald Armstrong
    • 1
  1. 1.Mars HillU.S.A.

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-322-0
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-321-3
  • Online ISBN 978-1-60761-322-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book