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  • Book
  • © 2009

Proteomics

Methods and Protocols

Editors:

  • Comprised of both overview articles and detailed protocols for core proteomics techniques

  • Covers the hot topics in proteomics research, such as relative and absolute protein quantification, organellar and membrane studies as well as PTM and protein interaction analysis

  • Contains method descriptions for both beginners and advanced proteomics researchers on electrophoresis, chromatography, and mass spectrometry

  • Provides guidance on proteomics methods from sample preparation to bioinformatical data interpretation written by experts in the respective fields

Part of the book series: Methods in Molecular Biology (MIMB, volume 564)

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USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-1-60761-157-8
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  • Tax calculation will be finalised during checkout
Softcover Book
USD 179.99
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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
  2. Introduction

    1. Front Matter

      Pages 1-1
    2. Introduction to Proteomics

      • Friedrich Lottspeich
      Pages 3-10
  3. Electrophorestic Separations

    1. Front Matter

      Pages 12-12
    2. High-Resolution Two-Dimensional Electrophoresis

      • Walter Weiss, Angelika Görg
      Pages 13-32
    3. Non-classical 2-D Electrophoresis

      • Jacqueline Burré, Ilka Wittig, Hermann Schägger
      Pages 33-57
    4. Protein Detection and Quantitation Technologies for Gel-Based Proteome Analysis

      • Walter Weiss, Florian Weiland, Angelika Görg
      Pages 59-82
  4. Mass Spectrometry and Tandem Mass Spectrometry Applications

    1. Front Matter

      Pages 84-84
    2. MALDI MS

      • Rainer Cramer
      Pages 85-103
    3. A Newcomer’s Guide to Nano-Liquid-Chromatography of Peptides

      • Thomas Fröhlich, Georg J. Arnold
      Pages 123-141
    4. Multidimensional Protein Identification Technology

      • Katharina Lohrig, Dirk Wolters
      Pages 143-153
    5. Characterization of Platelet Proteins Using Peptide Centric Proteomics

      • Oliver Simon, Stefanie Wortelkamp, Albert Sickmann
      Pages 155-171
    6. Identification of the Molecular Composition of the 20S Proteasome of Mouse Intestine by High-Resolution Mass Spectrometric Proteome Analysis

      • Reinhold Weber, Regina Preywisch, Nikolay Youhnovski, Marcus Groettrup, Michael Przybylski
      Pages 173-186
  5. Quantitative Proteomics

    1. Front Matter

      Pages 188-188
    2. Liquid Chromatography–Mass Spectrometry-Based Quantitative Proteomics

      • Michael W. Linscheid, Robert Ahrends, Stefan Pieper, Andreas Kühn
      Pages 189-205
  6. Interpretation of Mass Spectrometry Data

    1. Front Matter

      Pages 244-244
    2. Algorithms and Databases

      • Lennart Martens, Rolf Apweiler
      Pages 245-259

About this book

Since the development of the concept of "proteomics," great progress has granted deeper insight into the cellular network, but the enormous range of protein abundance, dynamics, and interactions leaves many further challenges in the field. In Proteomics: Methods and Protocols, experts in proteomic research have contributed detailed techniques, which present an overview of the contemporary challenges and possibilities in the various areas of proteomics, as well as cutting-edge protocols that provide concrete examples for successful proteome analysis. Written in the clear and concise Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easy-to-use, Proteomics: Methods and Protocols will serve as a valuable reference book for laboratories around the world and raise interest in the complex and vital field of proteomics research.

Keywords

  • Cellular networks
  • Electrophorestic separations
  • Escherichia coli
  • Organelle
  • Protein interactions
  • Protein modifications
  • Proteomics
  • Quantitative and subcellular proteomics
  • Tandem mass spectrometry
  • membrane proteins
  • molecular biology
  • protein complexes

Bibliographic Information

  • Book Title: Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Jörg Reinders, Albert Sickmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-157-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-60761-156-1

  • Softcover ISBN: 978-1-62703-831-7

  • eBook ISBN: 978-1-60761-157-8

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 441

  • Topics: Protein Biochemistry, Biochemistry

Buying options

eBook
USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-1-60761-157-8
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 179.99
Price excludes VAT (USA)
Hardcover Book
USD 179.99
Price excludes VAT (USA)