Protocols for Adult Stem Cells

  • Irina M. Conboy
  • David V. Schaffer
  • Mary Helen Barcellos-Hoff
  • Song Li

Part of the Methods in Molecular Biology™ book series (MIMB, volume 621)

Table of contents

  1. Front Matter
    Pages i-x
  2. Rodrigo Fernandez-Gonzalez, Irineu Illa-Bochaca, Dawne N. Shelton, Bryan E. Welm, Mary Helen Barcellos-Hoff, Carlos Ortiz-de-Solorzano
    Pages 1-28
  3. Irineu Illa-Bochaca, Rodrigo Fernandez-Gonzalez, Dawne N. Shelton, Bryan E. Welm, Carlos Ortiz-de-Solorzano, Mary Helen Barcellos-Hoff
    Pages 29-47
  4. Dawne N. Shelton, Rodrigo Fernandez-Gonzalez, Irineu Illa-Bochaca, Carlos Ortiz-de-Solorzano, Mary Helen Barcellos-Hoff, Bryan E. Welm
    Pages 49-55
  5. Joseph Peltier, Brandi K. Ormerod, David V. Schaffer
    Pages 57-63
  6. Joseph Peltier, Smita Agrawal, Matthew J. Robertson, David V. Schaffer
    Pages 65-87
  7. Matthew J. Robertson, Joseph Peltier, David V. Schaffer
    Pages 89-102
  8. Joseph Peltier, David V. Schaffer
    Pages 103-116
  9. Jennifer S. Park, Craig Hashi, Song Li
    Pages 117-137
  10. Michael J. Conboy, Irina M. Conboy
    Pages 149-163
  11. Michael J. Conboy, Massimiliano Cerletti, Amy J. Wagers, Irina M. Conboy
    Pages 165-173
  12. Back Matter
    Pages 203-207

About this book

Introduction

The study of adult stem cells has surged in recent years. Because they are responsible for the body’s natural ability to fight diseases, heal and recover, or fail and succumb to various maladies, it has become increasingly important to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. In Protocols for Adult Stem Cells, expert researchers present a variety of methods for studying five types of clinically-relevant mammalian stems cells: mammary, nerve, skeletal muscle, endothelial and mesenchymal. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Chapters include information that will assist researchers in obtaining, characterizing and studying these cells or adapting them to the stem cells of choice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Critical and cutting edge, Protocols for Adult Stem Cells is an essential guide which provides groundbreaking and novel techniques certain to redefine the field of stem cell biology.

Keywords

Endothelial Mammalian Mammary Mesenchymal Muscle Nerve Vivo cell biology molecular biology myogenesis

Editors and affiliations

  • Irina M. Conboy
    • 1
  • David V. Schaffer
    • 2
  • Mary Helen Barcellos-Hoff
    • 3
  • Song Li
    • 4
  1. 1.Dept. BioengineeringUniversity of California, BerkeleyBerkeleyUSA
  2. 2.Helen Wills Neuroscience Inst.University of California, BerkeleyBerkeleyUSA
  3. 3.Lawrence Berkeley National Lab., Dept. Cancer BiologyUniversity of California, BerkeleyBerkeleyUSA
  4. 4.Dept. BioengineeringUniversity of California, BerkeleyBerkeleyUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-063-2
  • Copyright Information Springer Science + Business Media, LLC 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-062-5
  • Online ISBN 978-1-60761-063-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book