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Mitosis

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Andrew D. McAinsh

    1. Chromosome Segregation Lab., Marie Curie Research Institute, Oxted, Surrey, United Kingdom

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  • Provides an easily accessible reference volume for investigating mitosis put together by leading researchers in the field
  • Presents approaches in both mammalian and chicken cell systems as well as in model single-cell and multi-cellular organisms
  • Covers a broad range of biochemical, genetic and cell biological methods focused on dissecting specific mitotic processes
  • Contains up-to-date live cell imaging-based approaches to study dynamic mitotic events
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 545)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Knock-in and Knock-out: The Use of Reverse Genetics in Somatic Cells to Dissect Mitotic Pathways

    • Alexis R. Barr, Deborah Zyss, Fanni Gergely
    Pages 1-19

  3. Functional Dissection of Mitotic Regulators Through Gene Targeting in Human Somatic Cells

    • Eli Berdougo, Marie-Emilie Terret, Prasad V. Jallepalli
    Pages 21-37

  4. RNAi in Drosophila S2 Cells as a Tool for Studying Cell Cycle Progression

    • Mónica Bettencourt-Dias, Gohta Goshima
    Pages 39-62

  5. Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast

    • Marco Geymonat, Adonis Spanos, Steven Sedgwick
    Pages 63-80

  6. Hydrodynamic Analysis of Human Kinetochore Complexes During Mitosis

    • Sarah E. McClelland, Andrew D. McAinsh
    Pages 81-98

  7. Isolation of Protein Complexes Involved in Mitosis and Cytokinesis from Drosophila Cultured Cells

    • Pier Paolo D’Avino, Vincent Archambault, Marcin R. Przewloka, Wei Zhang, Ernest D. Laue, David M. Glover
    Pages 99-112

  8. Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines

    • Michael H.A. Schmitz, Daniel W. Gerlich
    Pages 113-134

  9. Electron Tomography of Microtubule End-Morphologies in C. elegans Embryos

    • Eileen O’Toole, Thomas Müller-Reichert
    Pages 135-144

  10. Dissecting Mitosis with Laser Microsurgery and RNAi in Drosophila Cells

    • António J. Pereira, Irina Matos, Mariana Lince-Faria, Helder Maiato
    Pages 145-164

  11. Fluorescence Imaging of the Centrosome Cycle in Mammalian Cells

    • Suzanna L. Prosser, Andrew M. Fry
    Pages 165-183

  12. Visualization of Fluorescence-Tagged Proteins in Fission Yeast: The Analysis of Mitotic Spindle Dynamics Using GFP-Tubulin Under the Native Promoter

    • Masamitsu Sato, Mika Toya, Takashi Toda
    Pages 185-203

  13. Analysing Kinetochore Function in Human Cells: Spindle Checkpoint and Chromosome Congression

    • Christiane Klebig, Alberto Toso, Satyarebala Borusu, Patrick Meraldi
    Pages 205-220

  14. Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts

    • Michael J. Emanuele, P. Todd Stukenberg
    Pages 221-232

  15. Live Cell Imaging of Kinetochore Capture by Microtubules in Budding Yeast

    • Kozo Tanaka, Tomoyuki U. Tanaka
    Pages 233-242

  16. The Spindle Checkpoint: Assays for the Analysis of Spindle Checkpoint Arrest and Recovery

    • Josefin Fernius, Kevin G. Hardwick
    Pages 243-258

  17. Measuring Proteolysis in Mitosis

    • Catherine Lindon, Barbara Di Fiore
    Pages 259-270

  18. An In Vitro Assay for Cdc20-Dependent Mitotic Anaphase-Promoting Complex Activity from Budding Yeast

    • Scott C. Schuyler, Andrew W. Murray
    Pages 271-285

  19. In Vitro Assays for the Anaphase-Promoting Complex/Cyclosome (APC/C) in Xenopus Egg Extracts

    • Hiroyuki Yamano, Michelle Trickey, Margaret Grimaldi, Yuu Kimata
    Pages 287-300

  20. Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and Degradation

    • Adam Williamson, Lingyan Jin, Michael Rape
    Pages 301-312
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Keywords

About this book

With the advent of modern methodology, the phenomenal complexity of the protein components and regulatory steps involved in mitosis has become approachable. In Mitosis: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate the mechanism of mitosis at the molecular level. Ideal for both experienced and novice scientists, the volume includes step-by-step methods covering inactivation of genes of interest, depletion of proteins of interest, biochemical and microscope-based techniques, and procedures to monitor and measure key mitotic processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Mitosis: Methods and Protocols combines imaging, biochemical, and genetic methodologies in order to best guide researchers in their attempts to better understand the crucial processes of mitotic regulation.

Reviews

From the reviews: “McAinsh … introduces mitosis as a phenomenally complicated process of the splitting of genetic material into two daughter cells that prevents genomic instability. Twenty chapters comprise this collection of laboratory protocols of up-to-date approaches and methods … used to investigate the mechanism of mitosis at the molecular level. … The volume is intended for graduate students and researchers in the field.” (SciTech Book News, March, 2010)

Editors and Affiliations

  • Chromosome Segregation Lab., Marie Curie Research Institute, Oxted, Surrey, United Kingdom

    Andrew D. McAinsh

Bibliographic Information

  • Book Title: Mitosis

  • Book Subtitle: Methods and Protocols

  • Editors: Andrew D. McAinsh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-993-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-60327-992-5Published: 29 June 2009

  • Softcover ISBN: 978-1-62703-850-8Published: 24 November 2014

  • eBook ISBN: 978-1-60327-993-2Published: 28 May 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 345

  • Topics: Cell Biology, Developmental Biology, Cell Cycle Analysis, Gene Expression

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