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The Nucleus

Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging

  • Ronald Hancock

Part of the Methods in Molecular Biology book series (MIMB, volume 464)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Physical and Mechanical Properties

  3. Chromatin and Transcription

    1. Front Matter
      Pages 27-27
    2. Petra Björk, Lars Wieslander
      Pages 29-54
    3. Catherine Schurra, Aaron Bensimon
      Pages 71-90
    4. Patrick T. C. van den Bogaard, Sanjay Tyagi
      Pages 91-103
    5. Dennis Lohr, Hongda Wang, Ralph Bash, Stuart M. Lindsay
      Pages 123-138
  4. The Nuclear Envelope

    1. Front Matter
      Pages 159-159
    2. Victor Shahin, Yvonne Ludwig, Hans Oberleithner
      Pages 161-180
    3. Aurelia Cassany, Larry Gerace
      Pages 181-205
    4. Richard D. Byrne, Vanessa Zhendre, Banafshé Larijani, Dominic L. Poccia
      Pages 207-223
  5. Modifications of Nuclear Proteins

    1. Front Matter
      Pages 225-225
    2. Ok-Kyong Park-Sarge, Kevin D. Sarge
      Pages 255-265
    3. Jean-Christophe Amé, Antoinette Hakmé, Delphine Quenet, Elise Fouquerel, Françoise Dantzer, Valérie Schreiber
      Pages 267-283
    4. Young-Mi Go, Jan Pohl, Dean P. Jones
      Pages 303-317
  6. Protein Dynamics in the Nucleus

    1. Front Matter
      Pages 319-319
    2. Stefanie Weidtkamp-Peters, Klaus Weisshart, Lars Schmiedeberg, Peter Hemmerich
      Pages 321-341
    3. Martin E. van Royen, Pascal Farla, Karin A. Mattern, Bart Geverts, Jan Trapman, Adriaan B. Houtsmuller
      Pages 363-385
  7. Imaging Methods

    1. Front Matter
      Pages 387-387
    2. Jacques Rouquette, Karl-Henning Kalland, Stanislav Fakan
      Pages 403-413
    3. Kashif Ahmed, Ren Li, David P. Bazett-Jones
      Pages 415-423
  8. Back Matter
    Pages 441-444

About this book

Introduction

Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development of the new, powerful biochemical and biophysical methodologies described here are essential in this quest. In The Nucleus, researchers from more than forty leading international laboratories describe state-of-the-art methods for isolating nuclei and their components and for studying their structure and activities, including some pathology-associated features. Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging presents biophysical approaches to study the mechanical properties of nuclei, together with a comprehensive range of imaging methods. These include FISH, FRAP, FRET, molecular beacons, fluorescence correlation spectroscopy, single molecule tracking, and combing DNA for optical microscopy, recognition imaging for atomic force microscopy, and hybridisation, tomography, and spectroscopic imaging for electron microscopy.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain lists of necessary materials and reagents, readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls.

The Nucleus, Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging provides a comprehensive collection of the cutting-edge methods making a major contribution to understanding the nucleus and its nanostructure today.

Keywords

Chromatin DNA FISH Imaging methods Nuclear envelope Nuclear proteins Protein dynamics RNA Transcription electron microscopy gene expression genes hybridization molecular biology saccharomyces cerevisiae

Editors and affiliations

  • Ronald Hancock
    • 1
  1. 1.Hôtel-Dieu HospitalLaval University Cancer Research CentreQuébecCanada

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-461-6
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-460-9
  • Online ISBN 978-1-60327-461-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site