Chromatin Immunoprecipitation Assays

Methods and Protocols

  • Philippe Collas

Part of the Methods in Molecular Biology book series (MIMB, volume 567)

Table of contents

  1. Front Matter
    Pages i-x
  2. Géraldine Goens, Dorina Rusu, Laurent Bultot, Jean-Jacques Goval, Juana Magdalena
    Pages 27-43
  3. Joel Nelson, Oleg Denisenko, Karol Bomsztyk
    Pages 45-57
  4. John Arne Dahl, Philippe Collas
    Pages 59-74
  5. Leif C. Lindeman, Linn T. Vogt-Kielland, Peter Aleström, Philippe Collas
    Pages 75-86
  6. Lorant Szekvolgyi, Laszlo Imre, Doan Xuan Quang Minh, Eva Hegedus, Zsolt Bacso, Gabor Szabo
    Pages 99-111
  7. Raphael Gottardo
    Pages 133-143
  8. Enrique M. Muro, Jennifer A. McCann, Michael A. Rudnicki, Miguel A. Andrade-Navarro
    Pages 145-154
  9. Mona Abed, Dorit Kenyagin-Karsenti, Olga Boico, Amir Orian
    Pages 155-169
  10. Alexey Gavrilov, Elvira Eivazova, Iryna Pirozhkova, Marc Lipinski, Sergey Razin, Yegor Vassetzky
    Pages 171-188
  11. Ales Obrdlik, Piergiorgio Percipalle
    Pages 215-235
  12. Jean-Jacques Goval, Juana Magdalena
    Pages 237-247
  13. Anita L. Sørensen, Philippe Collas
    Pages 249-262
  14. Back Matter
    Pages 263-267

About this book

Introduction

Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easy-to-use, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cell’s biology.

Keywords

ChIP DNA DNA microarray Genome-wide datasets High-throughput sequencing Protein binding SNP Termination chromosome genetics molecular biology transcription

Editors and affiliations

  • Philippe Collas
    • 1
  1. 1.Institute of Basic Medical Sciences, Dept. BiochemistryUniversity of OsloOsloNorway

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-414-2
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-413-5
  • Online ISBN 978-1-60327-414-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book