Overview
- Includes two comprehensive review chapters and twenty-five detailed method chapters
- Provides state-of-the-art methodologies
- Covers expression, purification, detection, and assay of MMPs and TIMPs
- Details many essential techniques in one easily accessible volume
- Updated and improved second edition
- Chapters include the 'tricks of the trade' written by leading researchers
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 622)
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About this book
Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms. Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field. Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Matrix Metalloproteinase Protocols, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume.
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Keywords
Table of contents (27 protocols)
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MMPs and TIMPs: An Overview
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Expression and Purification of MMPs and TIMPs
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Detection of MMPs and TIMPs
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Assay of MMP and TIMP Activities
Reviews
Editors and Affiliations
Bibliographic Information
Book Title: Matrix Metalloproteinase Protocols
Editors: Ian M. Clark
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-299-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2010
Hardcover ISBN: 978-1-60327-298-8Published: 04 March 2010
Softcover ISBN: 978-1-4939-5622-7Published: 23 August 2016
eBook ISBN: 978-1-60327-299-5Published: 05 February 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XII, 492
Topics: Biochemistry, general, Science, Humanities and Social Sciences, multidisciplinary, Enzymology