Therapeutic Applications of RNAi

Methods and Protocols

  • Cristina M. Rondinone
  • John F. Reidhaar-Olson

Part of the Methods in Molecular Biology™ book series (MIMB, volume 555)

Table of contents

  1. Front Matter
    Pages i-x
  2. Muriel Golzio, Laurent Mazzolini, Aurélie Paganin-Gioanni, Justin Teissié
    Pages 15-27
  3. Lingegowda S. Mangala, Hee Dong Han, Gabriel Lopez-Berestein, Anil K. Sood
    Pages 29-42
  4. Ralph A. Tripp, Stephen Mark Tompkins
    Pages 43-61
  5. Flaminia Catteruccia, Elena A. Levashina
    Pages 63-75
  6. Morten Østergaard Andersen, Kenneth Alan Howard, Jørgen Kjems
    Pages 77-86
  7. Daniel H. Kim, John J. Rossi
    Pages 119-125
  8. Kenneth J. Dery, Veronica Gusti, Shikha Gaur, John E. Shively, Yun Yen, Rajesh K. Gaur
    Pages 127-144
  9. Back Matter
    Pages 145-148

About this book


The phenomenon of RNA interference has rapidly moved from groundbreaking scientific discovery to promising therapeutic approach. However, even as RNAi-based drugs enter the clinic, significant challenges remain, particulary in the area of delivery. Therapeutic Applications of RNAi provides detailed protocols in key areas of current focus, including testing of delivery vehicles, identification of appropriate model systems, and evaluation of the effects of RNAi in vivo. Produced by a team of internationally renowned authors, the volume describes the therapeutic applications of RNAi and potential pitfalls in oncology, viral infections and CNS disease, using a variety of delivery methods, including liposomes, peptide-based nanoparticles, polycationic polymers, and viral vehicles. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Therapeutic Applications of RNAi is an ideal guide for scientists attempting to solve the numerous challenges in this field and revolutionize the treatment of disease.


CNS disease Liposomes Oncology Peptide-based nanoparticles RNA RNA interference Viral infections siRNA siRNA delivery transcription

Editors and affiliations

  • Cristina M. Rondinone
  • John F. Reidhaar-Olson

There are no affiliations available

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-294-0
  • Online ISBN 978-1-60327-295-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site