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  • © 1996

The Protein Protocols Handbook

Editors:

Part of the book series: Springer Protocols Handbooks (SPH)

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  • ISBN: 978-1-60327-259-9
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Table of contents (144 protocols)

  1. Front Matter

    Pages i-xviii
  2. Quantitation of Proteins

    1. Front Matter

      Pages 1-1
    2. Protein Determination by UV Absorption

      • Alastair Aitken, Michèle Learmonth
      Pages 3-6
    3. The Lowry Method for Protein Quantitation

      • Jakob H. Waterborg, Harry R. Matthews
      Pages 7-9
    4. The Bradford Method for Protein Quantitation

      • Nicholas J. Kruger
      Pages 15-20
    5. Ultrafast Protein Determinations Using Microwave Enhancement

      • Robert E. Akins, Rocky S. Tuan
      Pages 21-28
    6. Quantitation of Tryptophan in Proteins

      • Alastair Aitken, Michèle Learmonth
      Pages 29-31
    7. Protein Quantitation Using Flow Cytometry

      • F. Andrew Ray, Thomas D. Friedrich, Judith Laffin, John M. Lehman
      Pages 33-38
    8. Copper Iodide Staining of Proteins on Solid Phases

      • Douglas D. Root, Kuan Wang
      Pages 39-43
  3. Electrophoresis of Proteins and Peptides and Detection in Gels

    1. Front Matter

      Pages 49-49
    2. Acid-Urea-Triton Polyacrylamide Gels for Histones

      • Jakob H. Waterborg
      Pages 91-100

About this book

In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.

Keywords

  • chemistry
  • protein
  • proteins

Reviews

The Protein Protocols Handbook has arrived at the right place at the right time.-Trends in Cell Biology

Editors and Affiliations

  • University of Hertfordshire, Hatfield, UK

    John M. Walker

Bibliographic Information

  • Book Title: The Protein Protocols Handbook

  • Editors: John M. Walker

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-1-60327-259-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media LLC 1996

  • eBook ISBN: 978-1-60327-259-9

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XLIV, 809

  • Topics: Biochemistry

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • ISBN: 978-1-60327-259-9
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout