Inositol Phosphates and Lipids

Methods and Protocols

  • Christopher J. Barker

Part of the Methods in Molecular Biology book series (MIMB, volume 645)

Table of contents

  1. Front Matter
    Pages i-x
  2. Christopher J. Barker, Christopher Illies, Per-Olof Berggren
    Pages 21-46
  3. Andrew J. Letcher, Michael J. Schell, Robin F. Irvine
    Pages 61-71
  4. Cristina Azevedo, Adam Burton, Matthew Bennett, Sara Maria Nancy Onnebo, Adolfo Saiardi
    Pages 73-85
  5. J. Kent Werner Jr., Traci Speed, Rashna Bhandari
    Pages 87-102
  6. Andreas Nagel, Christopher J. Barker, Per-Olof Berggren, Christopher Illies
    Pages 123-131
  7. Akihiro Mizutani, Katsuhiro Kawaai, Chihiro Hisatsune, Hideaki Ando, Takayuki Michikawa, Katsuhiko Mikoshiba
    Pages 133-141
  8. Roberta Fiume, Gabriella Teti, Irene Faenza, Lucio Cocco
    Pages 143-164
  9. Anne Wuttke, Olof Idevall-Hagren, Anders Tengholm
    Pages 219-235
  10. Back Matter
    Pages 237-239

About this book


Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In Inositol Phosphates and Lipids: Methods and Protocols, expert researchers introduce the basic methodological tools to measure inositol lipids and phosphates and also describe new approaches that have become available in the last 10 years, including RNA-silencing and the use of fluorescently labeled PH-domains to measure inositides in real-time in live cells, new sensitive methods to measure mass of both phosphates and lipids, as well as protocols involving inositol pyrophosphates. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, detailing tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Inositol Phosphates and Lipids: Methods and Protocols compiles many of the techniques that underscore phosphorylated inositol cell biology in one convenient guide.


Cellular regulation Fluorescently labeled PH-domains IP3 receptor Inositol pyrophosphates Intracellular calcium Phosphorylated inositols Polyphosphates RNA-silencing biology cell biology molecular biology signal transduction

Editors and affiliations

  • Christopher J. Barker
    • 1
  1. 1.Dept. Molecular Medicine & Surgery, Rolf Luft Research Ctr. for DiabetesKarolinska University Hospital SolnaStockholmSweden

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-174-5
  • Online ISBN 978-1-60327-175-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site