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  • © 2009

Bacteriophages

Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions

  • Examines tried-and-true methods along with the most cutting-edge protocols

  • Ideal for both professionals and newcomers due to convenient, easy-to-use style

  • Designed for modular use, providing a potential wide array of techniques based on the combination of the methods described

Part of the book series: Methods in Molecular Biology (MIMB, volume 501)

Buying options

eBook USD 189.00
Price excludes VAT (USA)
  • ISBN: 978-1-60327-164-6
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 249.00
Price excludes VAT (USA)
Hardcover Book USD 249.99
Price excludes VAT (USA)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xxii
  2. Bacteriophage Isolation

    1. Front Matter

      Pages 1-1
    2. Methods for the Isolation of Viruses from Environmental Samples

      • K. Eric Wommack, Kurt E. Williamson, Rebekah R. Helton, Shellie R. Bench, Danielle M. Winget
      Pages 3-14
    3. Bacteriophage Enrichment from Water and Soil

      • Rohan Van Twest, Andrew M. Kropinski
      Pages 15-21
    4. Isolation of Phage via Induction of Lysogens

      • Ra’l R. Raya, Elvira M. H’bert
      Pages 23-32
    5. Isolation of Viruses from High Temperature Environments

      • Jennifer Fulton, Trevor Douglas, and Mark Young
      Pages 43-54
    6. Isolation of Novel Large and Aggregating Bacteriophages

      • Philip Serwer, Shirley J. Hayes, Julie A. Thomas, Borries Demeler, Stephen C. Hardies
      Pages 55-66
  3. Bacteriophage Characterization

    1. Front Matter

      Pages 67-67
    2. Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay

      • Andrew M. Kropinski, Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr, Roger P. Johnson
      Pages 69-76
    3. Enumeration of Bacteriophages by the Direct Plating Plaque Assay

      • Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr, Roger P. Johnson
      Pages 77-80
    4. Enumeration of Bacteriophages Using the Small Drop Plaque Assay System

      • Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr, Roger P. Johnson
      Pages 81-85
    5. Determination of Virus Abundance by Epifluorescence Microscopy

      • Alice C. Ortmann, Curtis A. Suttle
      Pages 87-95
    6. Enumeration of Bacteriophages Using Flow Cytometry

      • Corina P.D. Brussaard
      Pages 97-111
    7. Basic Phage Electron Microscopy

      • Hans-W. Ackermann
      Pages 113-126
    8. Phage Classification and Characterization

      • Hans-W. Ackermann
      Pages 127-140
    9. Phage Host Range and Efficiency of Plating

      • Elizabeth Kutter
      Pages 141-149
    10. Bacteriophage Plaques: Theory and Analysis

      • Stephen T. Abedon, John Yin
      Pages 161-174

About this book

Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques.  With its well-organized modular design, Volume 1: Isolation, Characterization, and Interactions examines a multitude of topics, including the isolation of phages, morphological and molecular characterization, and interaction with bacteria.  Written in the highly successful Methods in Molecular Biology™ series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.

Keywords

  • Bacteria
  • Microbial ecology
  • Microbial evolution
  • Morphology
  • Pathogenicity
  • Termination
  • electron microscopy
  • molecular biology

Reviews

From the reviews:

"This book apart from the numerous texts recently published in the area of bacteriophage biology is the focus on the methodology used in this field. … This volume focuses largely on the classical protocols - as described by the editors … . This book should prove to be a valuable resource to even the experienced phage biologist and an essential reference book for postgraduate students and scientists new to the field of phage biology." (Sophie Foley, Microbiology Today, July, 2009)

Editors and Affiliations

  • University of Leicester, UK

    Martha R.J. Clokie

  • Public Health Agency of Canada, Guelph, Canada

    Andrew M. Kropinski

Bibliographic Information

  • Book Title: Bacteriophages

  • Book Subtitle: Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions

  • Editors: Martha R.J. Clokie, Andrew M. Kropinski

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-164-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-58829-682-5

  • Softcover ISBN: 978-1-61737-715-0

  • eBook ISBN: 978-1-60327-164-6

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXII, 308

  • Number of Illustrations: 41 b/w illustrations

  • Topics: Biochemistry, Microbiology, Bacteria, Biomedical Research

Buying options

eBook USD 189.00
Price excludes VAT (USA)
  • ISBN: 978-1-60327-164-6
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 249.00
Price excludes VAT (USA)
Hardcover Book USD 249.99
Price excludes VAT (USA)