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Transgenesis Techniques

Principles and Protocols

  • Elizabeth J. Cartwright

Part of the Methods in Molecular Biology book series (MIMB, volume 561)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Transgenesis in Various Model Systems

    1. Front Matter
      Pages 1-1
    2. Leonie Ringrose
      Pages 3-19
    3. Matthias Rieckher, Nikos Kourtis, Angela Pasparaki, Nektarios Tavernarakis
      Pages 21-39
    4. Maximiliano L. Suster, Hiroshi Kikuta, Akihiro Urasaki, Kazuhide Asakawa, Koichi Kawakami
      Pages 41-63
    5. Jana Loeber, Fong Cheng Pan, Tomas Pieler
      Pages 65-72
    6. Jean Cozzi, Ignacio Anegon, Valérie Braun, Anne-Catherine Gross, Christel Merrouche, Yacine Cherifi
      Pages 73-88
  3. Transgenesis in the Mouse

    1. Front Matter
      Pages 90-90
    2. James Gulick, Jeffrey Robbins
      Pages 91-104
    3. Wendy J.K. Gardiner, Lydia Teboul
      Pages 111-126
    4. J. Simon, C. Arthur, Victoria A. McGuire
      Pages 127-144
    5. Melanie Ullrich, Kai Schuh
      Pages 145-159
    6. Ivana Barbaric, T. Neil Dear
      Pages 161-184
    7. Roland H. Friedel
      Pages 185-197
    8. Anne Plück, Christian Klasen
      Pages 199-217
    9. Anne Plück, Christian Klasen
      Pages 219-229
    10. Anne Plück, Christian Klasen
      Pages 231-243
    11. Marie-Christine Birling, Françoise Gofflot, Xavier Warot
      Pages 245-263
    12. Xin Wang
      Pages 265-273
    13. Elizabeth J. Cartwright
      Pages 275-283
    14. Lucie Vizor, Sara Wells
      Pages 285-299
  4. Back Matter
    Pages 333-335

About this book

Introduction

One of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced. In Transgenesis Techniques: Principles and Protocols, Third Edition, experts in the field fully update and expand upon the previous edition in order to detail the transgenic techniques currently used to modify the genome.  The volume explores classic procedures to genetically modify mice and other model organisms, as well as cutting-edge practices involving microinjection, site-specific recombination systems, cryopreservation, and many other topics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and state-of-the-art, Transgenesis Techniques: Principles and Protocols, Third Edition is a comprehensive practical guide to the generation of transgenic animals and an invaluable source of information in any lab currently involved in transgenic techniques.

Keywords

DNA Mouse models Protein-coding genes Transgenic animals cryopreservation genes genome molecular biology

Editors and affiliations

  • Elizabeth J. Cartwright

There are no affiliations available

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-019-9
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-018-2
  • Online ISBN 978-1-60327-019-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site