Plant MicroRNAs

Methods and Protocols

  • Blake C. Meyers
  • Pamela J. Green

Part of the Methods in Molecular Biology book series (MIMB, volume 592)

Table of contents

  1. Front Matter
    Pages i-x
  2. Matthew W. Jones-Rhoades
    Pages 19-30
  3. Monica Accerbi, Skye A. Schmidt, Emanuele De Paoli, Sunhee Park, Dong-Hoon Jeong, Pamela J. Green
    Pages 31-50
  4. Noah Fahlgren, James C. Carrington
    Pages 51-57
  5. Michael J. Axtell
    Pages 59-70
  6. Rebecca Schwab, Stephan Ossowski, Norman Warthmann, Detlef Weigel
    Pages 71-88
  7. Kan Nobuta, Kevin McCormick, Mayumi Nakano, Blake C. Meyers
    Pages 89-106
  8. Cheng Lu, Frédéric Souret
    Pages 107-125
  9. Zoltán Havelda
    Pages 127-136
  10. Bin Yu, Xuemei Chen
    Pages 137-148
  11. Molly Megraw, Artemis G. Hatzigeorgiou
    Pages 149-161
  12. Gayathri Mahalingam, Blake C. Meyers
    Pages 163-181
  13. Florence Jay, Jean-Pierre Renou, Olivier Voinnet, Lionel Navarro
    Pages 183-202
  14. Dong-Hoon Jeong, Marcelo A. German, Linda A. Rymarquis, Shawn R. Thatcher, Pamela J. Green
    Pages 203-230
  15. Yukio Kurihara, Yuichiro Watanabe
    Pages 231-241
  16. Felipe F. de Felippes, Detlef Weigel
    Pages 255-264
  17. Back Matter
    Pages 265-267

About this book

Introduction

MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects.  In Plant MicroRNAs: Methods and Protocols, experts in the field present chapters that focus on the identification, validation, and characterization of the miRNA class of RNAs, and address important aspects about heterochromatic small interfering RNAs.  In addition, the methods contained in this volume emphasize miRNA analyses, but also include ways to distinguish one class of small RNAs from another.  As a volume in the highly successful Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easy to use, Plant MicroRNAs: Methods and Protocols provides the research community with a set of protocols that will help advance vital miRNA research for all plant species, both in typical model species and non-model species alike.

Keywords

Arabidopsis Evolution Heterochromatic siRNAs Post-transcriptional regulatory effects Promoter RNA purification Regulation Transcriptome arabidopsis thaliana bioinformatics conservation genes miRNAs molecular biology transcription

Editors and affiliations

  • Blake C. Meyers
    • 1
  • Pamela J. Green
    • 2
  1. 1.Delaware Biotechnology InstituteUniversity of DelawareNewarkU.S.A.
  2. 2.Delaware Biotechnology InstituteUniversity of DelawareNewarkU.S.A.

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-005-2
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-004-5
  • Online ISBN 978-1-60327-005-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book