Overview
- Contains step-by-step instructions that ensure successful results
- Provides tricks of the trade and notes on troubleshooting and avoiding known pitfalls
Part of the book series: Methods in Molecular Biology (MIMB, volume 422)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
About this book
The past decade has seen the emergence of a new field of scientific inquiry at the intersection of phylogenetics and genomics: phylogenomics. From one perspective, phylogenomics is defined as the use of large genomic data sets to aid in difficult phylogeny problems. Alternatively, phylogenomics may be described as the use of phylogeny and comparative analysis to infer processes of genome evolution. Regardless of how one defines the field, the two app- cations are intertwined. This volume is a collection of protocols and resources compiled by leading researchers in the field and describes many of the molecular methods and bioinformatics tools that have brought this field to fruition in recent years. Several chapters in this volume highlight the use of cytogenetic methods for characterizing the genomes of different species. Fluorescent in-situ hybridization (FISH) is a powerful tool for establishing chromosome homologies between divergent species. The broadest of these techniques is chromosome painting, which in recent years has been performed on members of nearly every order of eutherian mammals, and across marsupial and avian orders. FISH mapping of single copy clones (e. g. cDNAs, fosmids, and BACS) can provide ordered gene mapping from megabase-pair resolution on metaphase preparations down to exquisite kilobase-pair resolution detail with extended-fiber techniques. Other chapters highlight the construction and development of radiation-hybrid (RH) maps, now fueled by thousands of markers from either large scale BAC-end sequencing projects or survey-sequenced genomes.
Similar content being viewed by others
Keywords
Table of contents (15 protocols)
-
Front Matter
-
Back Matter
Editors and Affiliations
Bibliographic Information
Book Title: Phylogenomics
Editors: William J. Murphy
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-581-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-764-8Published: 16 April 2008
Softcover ISBN: 978-1-61737-764-8Published: 19 November 2010
eBook ISBN: 978-1-59745-581-7Published: 16 July 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 259
Number of Illustrations: 52 b/w illustrations
Topics: Biochemistry, general, Human Genetics