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DNA and RNA Profiling in Human Blood

Methods and Protocols

  • Peter Bugert

Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 496)

Table of contents

  1. Front Matter
    Pages i-xi
  2. DNA Profiling for Blood Cell Antigens

    1. Front Matter
      Pages 1-1
    2. Gianmarco Ferri, Susi Pelotti
      Pages 51-58
    3. Ying Lu, Julian Boehm, Lynn Nichol, Massimo Trucco, Steven Ringquist
      Pages 89-114
    4. Charles H. Lin, Joanne M. Yeakley, Timothy K. McDaniel, Richard Shen
      Pages 129-142
    5. Kirstin Finning, Pete Martin, Geoff Daniels
      Pages 143-157
  3. RNA Profiling in Blood Cells

    1. Front Matter
      Pages 159-159
    2. Colin J. H. Brenan, Douglas Roberts, James Hurley
      Pages 161-174
    3. Svenja Debey-Pascher, Daniela Eggle, Joachim L. Schultze
      Pages 175-210
    4. Martin Steinau, Mangalathu S. Rajeevan
      Pages 211-222
    5. Andrew Hillmann, Eimear Dunne, Dermot Kenny
      Pages 223-243
    6. Dmitri V. Gnatenko, John J. Dunn, John Schwedes, Wadie F. Bahou
      Pages 245-272
    7. Angelika Schedel, Nina Rolf
      Pages 273-283
    8. Ramiro Garzon
      Pages 293-298
    9. Louise Edvardsson, Tor Olofsson
      Pages 313-322
  4. Back Matter
    Pages 339-341

About this book

Introduction

Blood samples have consistently proven to be a key source of genetic material for a wide variety of diagnostic or research purposes.  In DNA and RNA Profiling in Human Blood: Methods and Protocols, leading international experts contribute both established and recently developed protocols for complex and high-throughput DNA and RNA profiling.  Divided into two thorough sections, the volume concentrates on DNA profiling for blood cell antigens through methods on high-throughput multiplex approaches and SNP typing, along with RNA profiling in blood cells addressing certain blood cell types such as platelets, reticulocytes, and megakaryocytes.  Written in the highly successful Methods in Molecular Biology™ series format, all of the chapters include brief introductions on the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as the Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, DNA and RNA Profiling in Human Blood: Methods and Protocols is an ideal guide to the molecular profiling approaches that have opened up this broad field of research and have shown great promise in the further identifying of disease markers in blood.

Keywords

Blood cell antigens Disease markers Genotyping High-throughput multiplex approaches Megakaryocytes Platelets Reticulocytes SNP typing

Editors and affiliations

  • Peter Bugert
    • 1
  1. 1.Institute of Transfusion Medicine and ImmunologyHeidelberg University Medical Faculty of MannheimGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-553-4
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-93-0
  • Online ISBN 978-1-59745-553-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site