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DNA Methylation

Methods and Protocols

  • Book
  • © 2009
  • Latest edition


  • Provides an easily accessible reference volume for DNA methylation technologies as written by leading experts in the field
  • Focuses on validated and breakthrough innovations for all major questions of development and disease-related DNA methylation research
  • Details methods usable for every laboratory in the world and ensures the rapid implementation of DNA methylation analysis in the laboratory
  • Comprehensive guide to take biomarkers identified through innovative technologies from the research lab to potential diagnostic and clinical applications
  • Covers a broad range of analysis and detection principles for DNA methylation research that can be individually combined to accommodate the researcher's need
  • Serves as the basis for future development of new high-performance and high-throughput assays for DNA methylation detection
  • Includes supplementary material:

Part of the book series: Methods in Molecular Biology (MIMB, volume 507)

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Table of contents (27 protocols)

  1. Global Methylation Levels

  2. Methods For Whole Genome Analysis Of Dna Methylation Patterns

  3. Gene-Specific Methylation Analysis


About this book

Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace. Due to this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updates, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts. This revision reflects contemporary study of the subject: the analysis of gene-specific DNA methylation patterns has been complemented by genome-wide approaches, and epigenomics takes a central place. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Methylation: Methods and Protocols, Second Edition is the perfect resource for scientists who wish to further our understanding of the molecular processes that determine the genomic DNA methylation landscape and for those who aim to identify and implement DNA methylation-based biomarkers for clinical and diagnostic applications.


From the reviews of the second edition:

"The 2nd edition … resulted in an extraordinarly helpful tool for lab practice and for developing multiples approaches in order to successfully perform these type of analysis. … authors made each chapter a valuable tool for beginners and senior investigators who want to explore DNA methylation analysis in their biological systems. … used for advanced researchers in order to complement their work with other methodologies. … This is definitively a volume that must be present at each lab working in this cutting-edge field … ." (Marcia Manterola, European Journal of Histochemistry, October, 2009)

Editors and Affiliations

  • CEA - Institut de Génomique, Evry, France

    Jörg Tost

Bibliographic Information

  • Book Title: DNA Methylation

  • Book Subtitle: Methods and Protocols

  • Editors: Jörg Tost

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-934115-61-9Published: 02 December 2008

  • Softcover ISBN: 978-1-61737-938-3Published: 01 December 2010

  • eBook ISBN: 978-1-59745-522-0Published: 06 November 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 392

  • Topics: Human Genetics, Gene Expression

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