Mitochondrial DNA

Methods and Protocols

  • Jeffrey A. Stuart

Part of the Methods in Molecular Biology™ book series (MIMB, volume 554)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Mitochondrial DNA Replication, Transcription, Translation, and Respiratory Complex Assembly

    1. Front Matter
      Pages 1-1
    2. Aurelio Reyes, Takehiro Yasukawa, Tricia J. Cluett, Ian J. Holt
      Pages 15-35
    3. Matthew J. Longley, Leslie A. Smith, William C. Copeland
      Pages 73-85
    4. Paola Loguercio Polosa, Stefania Deceglie, Marina Roberti, Maria Nicola Gadaleta, Palmiro Cantatore
      Pages 127-141
  3. Mitochondrial DNA Damage and Repair

    1. Front Matter
      Pages 163-163
    2. Adrian J. Lambert, Martin D. Brand
      Pages 165-181
    3. Sam W. Chan, Junjian Z. Chen
      Pages 183-197
    4. Melissa M. Page, Jeffrey A. Stuart
      Pages 213-231
    5. Christopher A. Koczor, Janet W. Snyder, Inna N. Shokolenko, Allison W. Dobson, Glenn L. Wilson, Susan P. LeDoux
      Pages 233-249
  4. Mitochondrial DNA Mutations

    1. Front Matter
      Pages 265-265

About this book

Introduction

Since the publication of the first edition, the number of unique heritable mtDNA mutations recognized as being associated with bioenergetic dysfunction, cell death and disease has grown. Likewise, our understanding of the basic biology of somatic mtDNA mutations continues to improve. In Mitochondrial DNA: Methods and Protocols, Second Edition, specialists from eight countries share their expertise, providing detailed protocols for studying many aspects of mtDNA. The volume is divided into three sections, which cover the transduction of information from mtDNA to functionally active respiratory complexes, mitochondrial reactive oxygen species (ROS) production as well as mtDNA damage and its repair, and the identification and quantification of heteroplasmic mtDNA mutations. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Mitrochondrial DNA: Methods and Protocols, Second Edition promises to aid researchers in further expanding our knowledge of this vital area of cell biology.

Keywords

DNA Damage and repair Disorders Heteroplasmic PCR Reactive oxygen species (ROS) Replication and transcription cell biology genetics human cell lines molecular biology mtDNA mutations saccharomyces cerevisiae tissue transcription

Editors and affiliations

  • Jeffrey A. Stuart
    • 1
  1. 1.Department of Biological SciencesBrock UniversityCpy CatharinesCanada

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-521-3
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-60-2
  • Online ISBN 978-1-59745-521-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book