Transgenic Maize

Methods and Protocols

  • M. Paul Scott

Part of the Methods in Molecular Biology™ book series (MIMB, volume 526)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Introduction

    1. Front Matter
      Pages 1-1
  3. Transformation Methods

    1. Front Matter
      Pages 21-21
    2. Kan Wang, Bronwyn Frame
      Pages 29-45
    3. Joseph F. Petolino, Nicole L. Arnold
      Pages 59-67
  4. Transgenic Maize in Research

    1. Front Matter
      Pages 69-69
    2. Amitabh Mohanty, Yan Yang, Anding Luo, Anne W. Sylvester, David Jackson
      Pages 71-89
  5. Analysis of Transgenic Plants

  6. Breeding with Transgenes

    1. Front Matter
      Pages 135-135
    2. Joan M. Peterson
      Pages 137-146
    3. Earl H. Bicar
      Pages 147-159
    4. Karla E. Vogel
      Pages 161-169

About this book

Introduction

As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes.  Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.

Keywords

Breeding Crops DNA Expression PCR Quantitative PCR Seed Termination Transformation Transgenes gene expression genes

Editors and affiliations

  • M. Paul Scott
    • 1
  1. 1.USDA-ARSCorn Insects and Crop Genetics Research UnitAmesUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-494-0
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-49-7
  • Online ISBN 978-1-59745-494-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book