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Serial Analysis of Gene Expression (SAGE)

Methods and Protocols

  • Kåre Lehmann Nielsen

Part of the Methods in Molecular Biology™ book series (MIMB, volume 387)

Table of contents

  1. Front Matter
    Pages i-x
  2. Experimental Procedures

    1. Front Matter
      Pages 1-1
    2. Annabeth Laursen Høgh, Kåre Lehmann Nielsen
      Pages 3-24
    3. Malali Gowda, Guo-Liang Wang
      Pages 25-38
    4. Anna M. Heidenblut
      Pages 39-54
    5. Hideo Matsumura, Monika Reuter, Detlev H. Krüger, Peter Winter, Günter Kahl, Ryohei Terauchi
      Pages 55-70
    6. Kåre Lehmann Nielsen
      Pages 71-80
    7. Kåre Lehmann Nielsen
      Pages 81-93
    8. Kåre Lehmann Nielsen
      Pages 109-119
  3. Tag Extraction and Analysis

    1. Front Matter
      Pages 121-121
    2. Jeppe Emmersen
      Pages 143-149
    3. Gerben J. Schaaf, Fred van Ruissen, Antoine van Kampen, Marcel Kool, Jan M. Ruijter
      Pages 151-168
    4. Fred van Ruissen, Gerben J. Schaaf, Marcel Kool, Frank Baas, Jan M. Ruijter
      Pages 169-183
    5. Haiyan Huang, Li Cai, Wing H. Wong
      Pages 185-198
  4. Back Matter
    Pages 205-208

About this book

Introduction

Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments. Of the several methods of genetic profiling available, only SAGE measures the expression of both known and unknown genes. SAGE studies encompass 50,000 tags and can provide detailed knowledge of the 2000 most highly expressed genes in the tissue sample. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world. Part 1 is dedicated to experimental procedures of SAGE and related methods including aRNA LongSAGE, SuperSAGE, DeepSAGE, and GMAT. Part 2 provides methods for extraction and filtration of tags, analysis of ditag populations, and completing statistically correct comparisons of gene expression profiles.

Comparative transcriptomics enables scientists to understand the underlying genetics of biological changes such as development, disease, crop yield, and resistance. SAGE analysis is also used to obtain unknown tags, which can be used as gene-specific primers in Rapid Amplification of cDNA Ends (RACE) reactions to generate full-length transcripts for cloning and sequencing. This book will be an indispensable tool for any lab engaged in genetic profiling and comparative transcriptomics, and will help many laboratories to successfully implement tag-based sequencing methods and procedures and obtain comprehensive, useful, and interpretative data.

Keywords

Filtration RNA gene expression genes genetics tissue transcription

Editors and affiliations

  • Kåre Lehmann Nielsen
    • 1
  1. 1.Department of Life SciencesAalborg UniversityAalborgDenmark

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-454-4
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-676-4
  • Online ISBN 978-1-59745-454-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site