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Microtubule Protocols

  • Book
  • © 2007

Overview

Editors:
  1. Jun Zhou

    1. Nankai University, Tianjin, China

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  • Includes methods for the development and discovery of novel agents that target microtubules
  • Includes readily reproducible protocols, tips for troubleshooting, and advice on avoiding common mistakes

Part of the book series: Methods in Molecular Medicine (MIMM, volume 137)

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About this book

Microtubules are essential components of the cytoskeleton, and play critical roles in a variety of cell processes, including cell shaping, intracellular tracking, cell division, and cell migration. Microtubule Protocols presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs. The straightforward presentation of readily reproducible protocols is a hallmark of the Methods in Molecular Medicine™ series, and is evident in this volume. Methods presented range from the purification and characterization of microtubule proteins, analysis of post-translational modifications of tubulin, and determination of microtubule structure, to the visualization of microtubule and spindle behavior, measurement of microtubule dynamics, and examination of microtubule-mediated cellular processes. Both basic scientists and clinical researchers will benefit from this collection of state-of-the-art protocols for microtubule research.

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Microtubules

    • Richard H. Wade
    Pages 1-16

  3. Large Scale Purification of Brain Tubulin With the Modified Weisenberg Procedure

    • José Manuel Andreu
    Pages 17-28

  4. Purification and Mass-Spectrometry Identification of Microtubule-Binding Proteins from Xenopus Egg Extracts

    • Vincent Gache, Patrice Waridel, Sylvie Luche, Andrej Shevchenko, Andrei V. Popov
    Pages 29-43

  5. Preparation and Characterization of Posttranslationally Modified Tubulins From Artemia franciscana

    • Paul A. O’Connell, Thomas H. MacRae
    Pages 45-63

  6. Studying the Structure of Microtubules by Electron Microscopy

    • Linda A. Amos, Keiko Hirose
    Pages 65-91

  7. Fluorescence Microscopy of Microtubules in Cultured Cells

    • Irina Semenova, Vladimir Rodionov
    Pages 93-102

  8. Measurements of Stathmin-Tubulin Interaction in Solution

    • Marie-France Carlier
    Pages 103-110

  9. Analysis of Microtubule Dynamics by Polarized Light

    • Rudolf Oldenbourg
    Pages 111-123

  10. Visualization of Spindle Behavior Using Confocal Microscopy

    • Adam I. Marcus
    Pages 125-137

  11. Live Cell Approaches for Studying Kinetochore-Microtubule Interactions in Drosophila

    • Daniel W. Buster, David J. Sharp
    Pages 139-160

  12. Analysis of Tubulin Transport in Nerve Processes

    • Andrey Tsvetkov, Sergey Popov
    Pages 161-173

  13. Analysis of Microtubule-Mediated Intracellular Viral Transport

    • Chunyong Liu, Min Liu, Jun Zhou
    Pages 175-180

  14. Xenopus Oocyte Wound Healing as a Model System for Analysis of Microtubule-Actin Interactions

    • Tong Zhang, Craig A. Mandato
    Pages 181-188

  15. Screening for Inhibitors of Microtubule-Associated Motor Proteins

    • Frank Kozielski, Salvatore DeBonis, Dimitrios A. Skoufias
    Pages 189-207

  16. Clinical Pharmacology and Use of Microtubule-Targeting Agents in Cancer Therapy

    • Amelia B. Zelnak
    Pages 209-234

  17. Studying Drug-Tubulin Interactions by X-Ray Crystallography

    • Audrey Dorleans, Marcel Knossow, Benoît Gigant
    Pages 235-243

  18. Characterizing Ligand-Microtubule Binding by Competition Methods

    • José Fernando DÍaz, Rubén MartÍnez Buey
    Pages 245-260

  19. Methods for Studying Vinca Alkaloid Interactions With Tubulin

    • Sharon Lobert, John J. Correia
    Pages 261-280

  20. High-Throughput Screening of Microtubule-Interacting Drugs

    • Susan L. Bane, Rudravajhala Ravindra, Anna A. Zaydman
    Pages 281-288
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Editors and Affiliations

  • Nankai University, Tianjin, China

    Jun Zhou

Bibliographic Information

  • Book Title: Microtubule Protocols

  • Editors: Jun Zhou

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1007/978-1-59745-442-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-642-9Published: 23 August 2007

  • Softcover ISBN: 978-1-61737-691-7Published: 10 November 2010

  • eBook ISBN: 978-1-59745-442-1Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: X, 305

  • Topics: Oncology, Cell Biology, Medicinal Chemistry, Cancer Research

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