Recombinant Proteins From Plants

Methods and Protocols

  • Loïc Faye
  • Véronique Gomord

Part of the Methods in Molecular Biology™ book series (MIMB, volume 483)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Christophe Sourrouille, Brian Marshall, David Liénard, Loïc Faye
    Pages 1-23
  3. Frank Sainsbury, Li Liu, George P. Lomonossoff
    Pages 25-39
  4. Marc-André D'Aoust, Pierre-Olivier Lavoie, Julie Belles-Isles, Nicole Bechtold, Michèle Martel, Louis-P. Vézina
    Pages 41-50
  5. Giuliana Medrano, Michael J. Reidy, Jianyun Liu, Jorge Ayala, Maureen C. Dolan, Carole L. Cramer
    Pages 51-67
  6. Thomas Rademacher, Elsa Arcalis, Eva Stoger
    Pages 69-87
  7. Bart Van Droogenbroeck, Kirsten De Wilde, Ann Depicker
    Pages 89-101
  8. Marta Ayala, Jorge Gavilondo, Meilyn Rodríguez, Alejandro Fuentes, Gil Enríquez, Lincidio Pérez et al.
    Pages 103-134
  9. Carole Plasson, Rémy Michel, David Lienard, Claude Saint-Jore-Dupas, Christophe Sourrouille, Ghislaine Grenier de March et al.
    Pages 145-161
  10. Margarita Torrent, Imma Llop-Tous, M. Dolors Ludevid
    Pages 193-208
  11. Guy Vancanneyt, Manuel Dubald, Werner Schröder, Jörg Peters, Johan Botterman
    Pages 209-221
  12. Muriel Bardor, Gleysin Cabrera, Johannes Stadlmann, Patrice Lerouge, José A. Cremata, Véronique Gomord et al.
    Pages 239-264
  13. Meriem Benchabane, Daniel Rivard, Cécile Girard, Dominique Michaud
    Pages 265-273
  14. Amine M. Badri, Karine Coenen, Louis-Philippe Vaillancourt, Goulet Charles, Dominique Michaud
    Pages 313-324

About this book

Introduction

The past decade has seen the emergence of plants as a viable alternative to the current production systems for therapeutic proteins. In Recombinant Proteins from Plants, expert researchers explore plants and their potential for the production of increasingly safe, high quality and biologically active complex recombinant pharmaceutical proteins. Chapters present several production platforms, focusing on the largest and most important group of biotechnological products in clinical trials: antibodies and their derived fragments with acknowledged potential for immunotherapy in humans. Due to the shortage of step by step protocols that can be used easily by beginners, this volume aims to fill the void with several chapters of detailed instructions for using the main plant expression systems. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Recombinant Proteins from Plants serves as an ideal guide for those new and seasoned plant scientists, pharmaceutical scientists, and molecular and cellular biologists interested in molecular pharming.

Keywords

Chloroplast Expression Molecular pharming Pharming Plant expression systems Plant pharming transgen

Editors and affiliations

  • Loïc Faye
    • 1
  • Véronique Gomord
    • 1
  1. 1.CNRSUniversité de RouenMont Saint AignanFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-407-0
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-978-9
  • Online ISBN 978-1-59745-407-0
  • Series Print ISSN 1064-3745
  • About this book