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The Nucleus

Volume 1: Nuclei and Subnuclear Components

  • Ronald Hancock

Part of the Methods in Molecular Biology book series (MIMB, volume 463)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. The Intranuclear Environment

    1. Front Matter
      Pages 1-1
    2. Santiago Schnell, Ronald Hancock
      Pages 3-19
  3. Isolation of Nuclei

  4. The Nucleolus

    1. Front Matter
      Pages 109-109
    2. Pavel Tchelidze, Hervé Kaplan, Adrien Beorchia, Marie-Françoise O’Donohue, Hélène Bobichon, Nathalie Lalun et al.
      Pages 137-158
  5. Intranuclear Structures

    1. Front Matter
      Pages 159-159
    2. Olga V. Zatsepina, Oxana O. Zharskaya, Andrei N. Prusov
      Pages 169-180
    3. Christine Iwahashi, Paul J. Hagerman
      Pages 181-190
  6. Interphase Chromosomes

    1. Front Matter
      Pages 203-203
    2. Marion Cremer, Florian Grasser, Christian Lanctôt, Stefan Müller, Michaela Neusser, Roman Zinner et al.
      Pages 205-239
    3. Antonius J. M. Matzke, Bruno Huettel, Johannes van der Winden, Marjori Matzke
      Pages 241-265
    4. Jiří Fajkus, Martina Dvořáčková, Eva Sýkorová
      Pages 267-296
    5. Przemek M. Krawczyk, Jan Stap, Ron A. Hoebe, Carel H. van Oven, Roland Kanaar, Jacob A. Aten
      Pages 309-320
  7. Back Matter
    Pages 321-323

About this book

Introduction

Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development of the new, powerful biochemical and biophysical methodologies described here are essential in this quest. In The Nucleus, researchers from more than forty leading international laboratories describe state-of-the-art methods for isolating nuclei and their components and for studying their structure and activities, including some pathology-associated features. Volume I: Nuclei and Subnuclear Structures presents an overview of features of the intranuclear environment, followed by the most recent procedures for isolating nuclei from a wide range of cell types including muscle cells, yeast, oocytes, cells with polytene nuclei, Arabidopsis, trypanosomes, and dinoflagellates. The latest methods are described for isolating and working with nucleoli, constitutive heterochromatin, pathology-associated inclusions, and chromatin and for examining glycosylation, sumoylation, and ADP-ribosylation of nuclear proteins. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain lists of necessary materials and reagents, readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls.

The Nucleus, Volume I: Nuclei and Subnuclear Structures is an essential reference for scientists who are working on our rapidly growing understanding of the cell nucleus and its activities.

Keywords

DNA FISH Interphase chromosomes Intranuclear structures Isolation Nucleolus RNA Telomere cell nucleus hybridization molecular biology proteins transcription

Editors and affiliations

  • Ronald Hancock
    • 1
  1. 1.Hôtel-Dieu HospitalLaval University Cancer Research CentreQuébecCanada

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-406-3
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-977-2
  • Online ISBN 978-1-59745-406-3
  • Series Print ISSN 1064-3745
  • Buy this book on publisher's site