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  • © 2007

Animal Cell Biotechnology

Methods and Protocols

Editors:

  • Chapters written by world-renowned experts

  • Details novel techniques for basic cultivation and complex techniques such as disposable bioreactors, hollow fiber cell culture and fixed bed reactors

  • Provides a state-of-the-art manual for setting up mammalian cell lines for production of biopharmaceuticals

  • Divided into sections to mimic the processes required for different stages of production

Part of the book series: Methods in Biotechnology (MIBT, volume 24)

Buying options

eBook USD 169.00
Price excludes VAT (USA)
  • ISBN: 978-1-59745-399-8
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 219.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xv
  2. Basic Techniques for Establishing Production Cell Lines

    1. Front Matter

      Pages 1-1
    2. Transduction Technologies

      • David A. Fluri, Martin Fussenegger
      Pages 3-21
    3. Cells for Gene Therapy and Vector Production

      • Christophe Delenda, Miguel Chillon, Anne-Marie Douar, Otto-Wilhelm Merten
      Pages 23-91
    4. Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments

      • Alexandra Dorn-Beineke, Stefanie Nittka, Michael Neumaier
      Pages 93-121
    5. DNA Fingerprinting and Characterization of Animal Cell Lines

      • Glyn N. Stacey, Ed Byrne, J. Ross Hawkins
      Pages 123-145
  3. Basic Cultivation Techniques

    1. Front Matter

      Pages 147-147
    2. Microcarrier Cell Culture Technology

      • Gerald Blüml
      Pages 149-178
    3. Cell Encapsulation

      • Holger Hübner
      Pages 179-191
    4. Tools for High-Throughput Medium and Process Optimization

      • Martin Jordan, Nigel Jenkins
      Pages 193-202
  4. Cell Characterization and Analysis

    1. Front Matter

      Pages 203-203
    2. Cell Counting and Viability Measurements

      • Michael Butler, Maureen Spearman
      Pages 205-222
    3. Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry

      • Silvia Carroll, Mariam Naeiri, Mohamed Al-Rubeai
      Pages 223-237
    4. Measurement of Apoptosis in Cell Culture

      • Adiba Ishaque, Mohamed Al-Rubeai
      Pages 285-299
    5. Metabolic Flux Estimation in Mammalian Cell Cultures

      • Chetan T. Goudar, Richard Biener, James M. Piret, Konstantin B. Konstantinov
      Pages 301-317
  5. Special Cultivation Techniques

    1. Front Matter

      Pages 319-319

About this book

Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation.

Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors.

Animal Cell Biotechnology: Methods and Protocols, Second Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Keywords

  • DNA
  • Downstream Processing
  • Expression
  • biotechnology
  • cell lines
  • gene therapy
  • genes
  • mammalian cell lines
  • metabolism
  • physiology

Editors and Affiliations

  • Institute for Bioprocess and Biochemical Engineering, Hamburg University of Technology, Hamburg, Germany

    Ralf Pörtner

Bibliographic Information

  • Book Title: Animal Cell Biotechnology

  • Book Subtitle: Methods and Protocols

  • Editors: Ralf Pörtner

  • Series Title: Methods in Biotechnology

  • DOI: https://doi.org/10.1007/978-1-59745-399-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-660-3

  • Softcover ISBN: 978-1-62703-963-5

  • eBook ISBN: 978-1-59745-399-8

  • Series ISSN: 1940-6061

  • Series E-ISSN: 1940-607X

  • Edition Number: 2

  • Number of Pages: XV, 512

  • Topics: Biotechnology, Cell Biology, Genetics and Genomics, Pharmacology

Buying options

eBook USD 169.00
Price excludes VAT (USA)
  • ISBN: 978-1-59745-399-8
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 219.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)