Overview
- Chapters written by world-renowned experts
- Details novel techniques for basic cultivation and complex techniques such as disposable bioreactors, hollow fiber cell culture and fixed bed reactors
- Provides a state-of-the-art manual for setting up mammalian cell lines for production of biopharmaceuticals
- Divided into sections to mimic the processes required for different stages of production
Part of the book series: Methods in Biotechnology (MIBT, volume 24)
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Table of contents (23 protocols)
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Front Matter
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Basic Techniques for Establishing Production Cell Lines
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Front Matter
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Basic Cultivation Techniques
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Front Matter
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Cell Characterization and Analysis
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Front Matter
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Special Cultivation Techniques
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Front Matter
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Keywords
About this book
Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation.
Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors.
Animal Cell Biotechnology: Methods and Protocols, Second Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
Editors and Affiliations
Bibliographic Information
Book Title: Animal Cell Biotechnology
Book Subtitle: Methods and Protocols
Editors: Ralf Pörtner
Series Title: Methods in Biotechnology
DOI: https://doi.org/10.1007/978-1-59745-399-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2007
Hardcover ISBN: 978-1-58829-660-3Published: 05 April 2007
Softcover ISBN: 978-1-62703-963-5Published: 04 November 2014
eBook ISBN: 978-1-59745-399-8Published: 05 February 2008
Series ISSN: 1940-6061
Series E-ISSN: 1940-607X
Edition Number: 2
Number of Pages: XV, 512
Topics: Biotechnology, Cell Biology, Animal Genetics and Genomics, Pharmacology/Toxicology