In Vitro Transcription and Translation Protocols

  • Guido Grandi

Part of the Methods in Molecular Biology™ book series (MIMB, volume 375)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Technologies

    1. Front Matter
      Pages 1-1
    2. Vladimir A. Shirokov, Aigar Kommer, Vyacheslav A. Kolb, Alexander S. Spirin
      Pages 19-55
    3. Christian Klammt, Daniel Schwarz, Volker Dötsch, Frank Bernhard
      Pages 57-78
    4. Suang Rungpragayphan, Tsuneo Yamane, Hideo Nakano
      Pages 79-94
    5. Tatsuya Sawasaki, Ryo Morishita, Mudeppa D. Gouda, Yaeta Endo
      Pages 95-106
    6. Elena Bossi, Maria Serena Fabbrini, Aldo Ceriotti
      Pages 107-131
  3. Applications

    1. Front Matter
      Pages 133-133
    2. Zene Matsuda, Mutsunori Iga, Kosuke Miyauchi, Jun Komano, Kazuhiro Morishita, Akihiko Okayama et al.
      Pages 135-149
    3. Yuichi Koga, Tsuneo Yamane, Hideo Nakano
      Pages 165-181
    4. Nathalie Norais, Ignazio Garaguso, Germano Ferrari, Guido Grandi
      Pages 183-209
    5. Stefano Giovannardi, Andrea Soragna, Simona Magagnin, Laura Faravelli
      Pages 235-255
  4. Back Matter
    Pages 273-276

About this book

Introduction

A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps.

Keywords

ATP Mutant Mutation PCR Translation genes genetic information molecular biology proteins transcription

Editors and affiliations

  • Guido Grandi
    • 1
  1. 1.Biochemistry and Molecular Biology UnitNovartis VaccinesSienaItaly

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-388-2
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-558-3
  • Online ISBN 978-1-59745-388-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book