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Cryopreservation and Freeze-Drying Protocols

  • Book
  • © 2007
  • Latest edition


  • Background and explanatory notes for each protocol
  • Methods for lyophilization of proteins
  • Cutting-edge methods for conservation and sustainable use of resources
  • New and novel techniques for preserving biological materials

Part of the book series: Methods in Molecular Biology (MIMB, volume 368)

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About this book

Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995.

This volume begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Detailed descriptions of materials required and stepwise protocols are presented for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources.

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Table of contents (23 protocols)


From the reviews of the second edition:

"...practical and reliable resource for the conservation and sustainable use of biological resources."-Anticancer Research

"This book is a welcome addition to the relatively sparse comprehensive reference publications on preservation methodologies which are currently available. It begins sensibly with a grounding in the principles involved in preservation, in logical and easy to follow steps. It is well laid out and covers a wide spectrum of cell/organism types in the one volume … ." (Peter Green, Molecular Biotechnology, December, 2007)

Editors and Affiliations

  • Culture Collection of Algae and Protozoa, Scottish Association for Marine Science, Dunstaffnage Marine Laboratory, Dunbeg, Argyll, UK

    John G. Day

  • Division of Cell Biology and Imaging, National Institute for Biological Standards and Control, Potters Bar, Herts, UK

    Glyn N. Stacey

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