Monoclonal Antibodies

Methods and Protocols

  • Maher Albitar

Part of the Methods in Molecular Biology book series (MIMB, volume 378)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Mariel Donzeau, Achim Knappik
    Pages 15-31
  3. Meg L. Flanagan, Robyn S. Arias, Peisheng Hu, Leslie A. Khawli, Alan L. Epstein
    Pages 33-52
  4. Mohammad Reza Sheikholeslami, Iman Jilani, Maher Albitar
    Pages 53-63
  5. Huai En Huang Chan, Iman Jilani, Richard Chang, Maher Albitar
    Pages 65-69
  6. Patrick Schroeter, Arnim Sablotzki, Dagmar Riemann
    Pages 71-81
  7. Huai En Huang Chan, Iman Jilani, Richard Chang, Maher Albitar
    Pages 83-90
  8. Rudolf Varro, Roy Chen, Homero Sepulveda, John Apgar
    Pages 125-152
  9. Huai En Huang Chan, Iman Jilani, Richard Chang, Maher Albitar
    Pages 153-157
  10. Huai En Huang Chan, Iman Jilani, Richard Chang, Maher Albitar
    Pages 167-174
  11. M. Michael Glovsky
    Pages 205-219
  12. Back Matter
    Pages 221-228

About this book

Introduction

Monoclonal Antibodies: Methods and Protocols examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting with opening chapters focusing on the gold standard method for generating mouse monoclonal antibodies through hybridoma technology, future methods for engineering recombinant and humanized antibodies, methods for engineering soluble Fc fusion protein, and the use of antibodies and flow cytometry in the quantification of cell signaling proteins.

Specific chapters describe how antibodies are used for the diagnosis and classification of hematologic diseases. Subsequent chapters examine the advantages and most recent advances of using bead-based immunoassays, including the ability of bead-based technology to multiplex and analyze several analytes simultaneously, and the use of beads in detecting fusion proteins resulting from chromosomal translocations. Concluding chapters provide additional examples of methodologies that employ monoclonal antibodies.

Monoclonal Antibodies: Methods and Protocols provides descriptions of methods that cover a wide spectrum of applications in the field of monoclonal antibodies. This field will continue to expand and provide new and innovative techniques, not only in the laboratory, but also as a basis that complements targeted therapy.

Keywords

Antigen antibody cell chromosome classification development diseases immune system leukemia lymphoma membrane protein proteins targeted therapy tumor

Editors and affiliations

  • Maher Albitar
    • 1
  1. 1.Department of HematopathologyQuest Diagnostics Nichols InstituteSan Juan Capistrano

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-323-3
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-567-5
  • Online ISBN 978-1-59745-323-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book