Single Cell Diagnostics

Methods and Protocols

  • Alan Thornhill

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 132)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Nobuki Nakamura, Katharina Ruebel, Long Jin, Xiang Qian, Heyu Zhang, Ricardo V. Lloyd
    Pages 11-18
  3. Paul N. Scriven, Caroline Mackie Ogilvie
    Pages 19-30
  4. Bernhard Zimmermann, Xiao Yan Zhong, Wolfgang Holzgreve, Sinuhe Hahn
    Pages 43-49
  5. Jill L. Maron, Kirby L. Johnson, Diana W. Bianchi
    Pages 51-63
  6. Karen V. Schowalter, Jolene Fredrickson, Alan R. Thornhill
    Pages 87-99
  7. Lucille Voullaire, Leeanda Wilton
    Pages 101-115
  8. Back Matter
    Pages 175-182

About this book

Introduction

The aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR) As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.

Keywords

DNA FISH Fluoreszenz in situ-Hybridisierung Microarray Mutation PCR Quantitative PCR Termination chromosome gene expression genes hybridization

Editors and affiliations

  • Alan Thornhill
    • 1
  1. 1.The London Bridge FertilityGynecology and Genetics CentreLondonUK

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-298-4
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-578-1
  • Online ISBN 978-1-59745-298-4
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • About this book