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Cancer Stem Cells

Methods and Protocols

  • Book
  • © 2009


  • Provides easy to follow protocols in this technically challenging field
  • Presents a cohesive review of a variety of methods to isolate, identify, and study cancer stem cells
  • Is a timely contribution in this nascent field and will level the playing field of investigators of cancer stem cells
  • Presents a comprehensive, step-by-step guide by leading researchers in the field of cancer stem cells
  • Brings to light methods that have been mired in mystery
  • Brings together divergent viewpoints and methods to isolate the same population of cells, from prospective isolation using surface markers to stem cell propagation assays

Part of the book series: Methods in Molecular Biology (MIMB, volume 568)

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About this book

Cancer is a devastating disease that affects millions of people in the world. Our the- pies for tumors have mostly been based on classical chemotherapy and antiproliferative treatments. More recently, directed therapies against a causative oncogene have led to prominent reduction in tumor rates for some cancers. For instance, chronic myelogenous leukemia that is due to a BCR–ABL translocation can be targeted with Gleevec. Despite these advances in individual tumors, a number of patients are treated for their primary tumors and ultimately relapse. Relapsing tumors can be due to resistance to chemotherapy or to antioncogene drugs. Another hypothesis is that heterogeneity in the tumor leads to an inability of classical chemotherapy to completely eradicate all cells of the tumor. This concept of heterogeneity has led some investigators to propose a cancer stem cell model. As patients are treated with chemotherapy, most of the dividing cells are killed, but this leaves a small subset of cells that have the ability to remake the entire tumor. These are cancer stem cells. They possess the signals of self-renewal and yet can also differentiate. If one could understand more about the cells that remain after classical chemotherapy or the cells that can remake the tumor among the heterogeneous population, this information would have a huge impact on our treatment of cancer.

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Table of contents (17 protocols)


From the reviews:

“This volume in the popular Methods in Molecular Biology series provides a state-of-the art compendium of research techniques and protocols for identifying and culturing rare populations of cancer stem cells. … The audience includes basic science and clinical researchers who are interested in stem cell biology as well as mechanisms of carcinogenesis and malignancy. The book also will be valued by biomedical scientists and clinicians interested in clinical oncology. … The topic is fascinating and clinically relevant.” (Bruce A. Fenderson, Doody’s Review Service, June, 2010)

Bibliographic Information

  • Book Title: Cancer Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: John S. Yu

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-58829-938-3Published: 17 August 2009

  • Softcover ISBN: 978-1-61779-753-8Published: 09 August 2012

  • eBook ISBN: 978-1-59745-280-9Published: 07 July 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 284

  • Number of Illustrations: 42 b/w illustrations, 16 illustrations in colour

  • Topics: Oncology, Cancer Research, Stem Cells, Cell Culture

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