Adenovirus Methods and Protocols

Volume 2: Ad Proteins, RNA Lifecycle, Host Interactions, and Phylogenetics

  • William S. M. Wold
  • Ann E. Tollefson

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 131)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Cristina Iftode, S. J. Flint
    Pages 1-14
  3. Paul M. Loewenstein, Chao-Zhong Song, Maurice Green
    Pages 15-31
  4. Gunnar Andersson, Ning Xu, Göran Akusjärvi
    Pages 47-61
  5. David A. Ornelles, Robin N. Broughton-Shepard, Felicia D. Goodrum
    Pages 83-101
  6. Peter Yaciuk
    Pages 103-111
  7. Anuj Gaggar, Dmitry Shayakhmetov, André Lieber
    Pages 141-155
  8. Maurice Green, Andrew Thorburn, Robert Kern, Paul M. Loewenstein
    Pages 157-185
  9. Michael Nevels, Thomas Dobner
    Pages 187-195
  10. Walter Doerfler
    Pages 197-211
  11. John J. Rux, Roger M. Burnett
    Pages 231-250
  12. Walter F. Mangel, William J. McGrath
    Pages 257-267

About this book

Introduction

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise.

Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.

Keywords

PCR RNA Splicing Termination Viruses Vivo genetics protein complexes proteins transcription

Editors and affiliations

  • William S. M. Wold
    • 1
  • Ann E. Tollefson
    • 1
  1. 1.Saint Louis University School of MedicineMissouri

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-277-9
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-901-7
  • Online ISBN 978-1-59745-277-9
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • About this book