Overview
- Records our progress to date in understanding the intricacies of Wnt signalling
- Includes assays for and models of both canonical and non-canonical Wnt signalling pathways
- Contributing authors are either current or past members of the main laboratories that have shaped the Wnt field
- Includes detailed methods as well as overviews, providing easy to follow protocols as well as a background/current status of the field
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 468)
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About this book
Since their discovery, Wnt signaling molecules have been shown to control key events in embryogenesis, to maintain tissue homeostasis in the adult and, when aberrantly activated, to promote human degenerative diseases and cancer, thus making them a vital area of study. Wnt Signaling: Methods and Protocols examines both biochemical assays and vertebrate and invertebrate model systems to provide a point of reference to current molecular protocols and the diverse model systems employed to study this important signaling pathway. In Volume 1, Pathway Methods and Mammalian Models, assays to measure activation of the diverse Wnt signaling pathways are explored, along with a selection of models and strategies used to study mammalian Wnt/FZD fuction, from protein-protein interaction and simple cell line models to organoid cultures and mouse models. As a volume in the highly successful Methods in Molecular Biologyâ„¢ series, chapters contain readily reproducible laboratory protocols, complete with lists of necessary equipment and reagents and the Notes section, which reveals helpful troubleshooting tips.
Comprehensive and cutting-edge, Wnt Signaling: Methods and Protocols collects the expertise and knowledge of many leaders in the field to produce this invaluable two-volume resource.
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Keywords
Table of contents (24 protocols)
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Front Matter
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Wnt Signaling Pathway Methods
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Canonical Wnt/FZD Signaling
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Non-Canonical Signaling
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Editors and Affiliations
Bibliographic Information
Book Title: Wnt Signaling
Book Subtitle: Volume 1: Pathway Methods and Mammalian Models
Editors: Elizabeth Vincan
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-249-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-912-3Published: 09 October 2008
Softcover ISBN: 978-1-61737-841-6Published: 20 January 2011
eBook ISBN: 978-1-59745-249-6Published: 21 December 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 336
Number of Illustrations: 74 b/w illustrations, 2 illustrations in colour
Topics: Cell Biology