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Table of contents

  1. Front Matter
    Pages i-xiii
  2. Raphael Sandaltzopoulos, Peter B. Becker
    Pages 11-25
  3. Carolina Pardo, Scott A. Hoose, Santhi Pondugula, Michael P. Kladde
    Pages 41-65
  4. Randall H. Morse
    Pages 93-108
  5. Daria A. Gaykalova, Olga I. Kulaeva, Vladimir A. Bondarenko, Vasily M. Studitsky
    Pages 109-123
  6. Hélène Gaillard, Ralf Erik Wellinger, Andrés Aguilera
    Pages 141-159
  7. Toshiki Tanaka, Dorota Halicka, Frank Traganos, Zbigniew Darzynkiewicz
    Pages 161-168
  8. Wasia Rizwani, Srikumar P. Chellappan
    Pages 203-216
  9. Marc Tini, Hina Naeem, Joseph Torchia
    Pages 235-247
  10. Kenji Kokura, Jia Fang
    Pages 249-261
  11. Shrikanth S. Gadad, Jayasha Shandilya, Venkatesh Swaminathan, Tapas K. Kundu
    Pages 263-278
  12. Zhigang Yuan, Natalie Rezai-Zadeh, Xiaohong Zhang, Edward Seto
    Pages 279-293
  13. Sean J. Yoder, Steven A. Enkemann
    Pages 367-381
  14. Sandeep Misri, Shruti Pandita, Tej K. Pandita
    Pages 395-410
  15. Back Matter
    Pages 411-416

About this book

Introduction

Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA.  In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function.  Written in the highly successful Methods in Molecular Biology™ series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology.

Keywords

Chromatin immunoprecipitation (ChIP) assays DNA DNA repair and replication Histones Nucleosomes Polymerase Chain Reaction Telomere hybridization molecular biology transcription

Editors and affiliations

  • Srikumar P. Chellappan
    • 1
  1. 1.Research InstituteH. Lee Moffitt Cancer Center &TampaU.S.A.

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-190-1
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-873-7
  • Online ISBN 978-1-59745-190-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site