Organelle Proteomics

  • Delphine Pflieger
  • Jean Rossier

Part of the Methods in Molecular Biology™ book series (MIMB, volume 432)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography–Tandem Mass Spectrometry

    1. Front Matter
      Pages 1-1
  3. Analysis of Organelles, Vesicles, and Protein Machineries

    1. Front Matter
      Pages 17-17
    2. Daniel Salvi, Norbert Rolland, Jacques Joyard, Myriam Ferro
      Pages 19-36
    3. Mark S. Lowenthal, Kathryn E. Howell, Christine C. Wu
      Pages 37-49
    4. Hans Zischka, Norbert Kinkl, Ralf J. Braun, Marius Ueffing
      Pages 51-64
    5. Thierry Rabilloud
      Pages 83-100
    6. Laurence Florens, Nadia Korfali, Eric C. Schirmer
      Pages 117-137
    7. Tsuneyoshi Horigome, Kazuhiro Furukawa, Kohei Ishii
      Pages 139-148
    8. Kiichi Fukui, Hideaki Takata, Susumu Uchiyama
      Pages 149-160
    9. Erik Alexandersson, Niklas Gustavsson, Katja Bernfur, Adine Karlsson, Per Kjellbom, Christer Larsson
      Pages 161-173
    10. Adele R. Blackler, Christine C. Wu
      Pages 185-197
    11. Markus Islinger, Gerhard Weber
      Pages 199-215
    12. Jacob Kennedy, Eugene C. Yi
      Pages 217-228
    13. Huiwen Zhang, Xiaolian Fan, Rick Bagshaw, Don J. Mahuran, John W. Callahan
      Pages 229-241
    14. Sylvie Kieffer– Jaquinod, Agnès Chapel, Jérôme Garin, Agnøs Journet
      Pages 243-258

About this book

Introduction

Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study.

Keywords

Bur Chloroplast Genome Genomics Mutant Organelle Protein Proteomics Termination chromosome molecular mechanisms proteins saccharomyces cerevisiae

Editors and affiliations

  • Delphine Pflieger
    • 1
    • 2
  • Jean Rossier
    • 1
  1. 1.Laboratoire de Neurobiologie et Diversité Cellulaire CNRS UMR 7637Ecole Supérieure de Physique et de Chimie IndustriellesFrance
  2. 2.Laboratoire d’Analyse et de Modélisation pour la Biologie et I’Environnement CNRS UMR 8587Université d’Evry Val d’EssonneEvryFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-028-7
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-779-2
  • Online ISBN 978-1-59745-028-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book