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Cell Imaging Techniques

  • Book
  • © 2006

Overview

Editors:
  1. Douglas J. Taatjes

    1. Department of Pathology, University of Vermont, Burlington

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    You can also search for this editor in PubMed   Google Scholar

  2. Brooke T. Mossman

    1. Department of Pathology, University of Vermont, Burlington

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 319)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Molecular Beacons

    • Diana P. Bratu
    Pages 1-14

  3. Second-Harmonic Imaging of Collagen

    • Guy Cox, Eleanor Kable
    Pages 15-35

  4. Visualizing Calcium Signaling in Cells by Digitized Wide-Field and Confocal Fluorescent Microscopy

    • Michael Wm. Roe, Jerome F. Fiekers, Louis H. Philipson, Vytautas P. Bindokas
    Pages 37-66

  5. Multifluorescence Labeling Techniques and Confocal Laser Scanning Microscopy on Lung Tissue

    • Maria Stern, Douglas J. Taatjes, Brooke T. Mossman
    Pages 67-76

  6. Evaluation of Confocal Microscopy System Performance

    • Robert M. Zucker
    Pages 77-135

  7. Quantitative Analysis of Atherosclerotic Lesion Composition in Mice

    • Marilyn P. Wadsworth, Burton E. Sobel, David J. Schneider, Wendy Tra, Hans van Hirtum, Douglas J. Taatjes
    Pages 137-152

  8. Applications of Microscopy to Genetic Therapy of Cystic Fibrosis and Other Human Diseases

    • Thomas O. Moninger, Randy A. Nessler, Kenneth C. Moore
    Pages 153-163

  9. Laser Scanning Cytometry

    • Piotr Pozarowski, Elena Holden, Zbigniew Darzynkiewicz
    Pages 165-192

  10. Near-Clinical Applications of Laser Scanning Cytometry

    • David A. Rew, Gerrit Woltmann, Davinder Kaur
    Pages 193-212

  11. Laser Capture Microdissection

    • Virginia Espina, John Milia, Glendon Wu, Stacy Cowherd, Lance A. Liotta
    Pages 213-229

  12. Analysis of Asbestos-Induced Gene Expression Changes in Bronchiolar Epithelial Cells Using Laser Capture Microdissection and Quantitative Reverse Transcriptase-Polymerase Chain Reaction

    • Christopher B. Manning, Brooke T. Mossman, Douglas J. Taatjes
    Pages 231-236

  13. New Approaches to Fluorescence In Situ Hybridization

    • Sabita K. Murthy, Douglas J. Demetrick
    Pages 237-259

  14. Microarray Image Scanning

    • Latha Ramdas, Wei Zhang
    Pages 261-273

  15. Near-Field Scanning Optical Microscopy in Cell Biology and Cytogenetics

    • Michael Hausmann, Birgit Perner, Alexander Rapp, Leo Wollweber, Harry Scherthan, Karl-Otto Greulich
    Pages 275-294

  16. Porosome

    • Bhanu P. Jena
    Pages 295-316

  17. Secretory Vesicle Swelling by Atomic Force Microscopy

    • Sang-Joon Cho, Bhanu P. Jena
    Pages 317-330

  18. Imaging and Probing Cell Mechanical Properties With the Atomic Force Microscope

    • Kevin D. Costa
    Pages 331-361

  19. Reflection Contrast Microscopy

    • F. A. Prins, I. Cornelese-ten Velde, E. de Heer
    Pages 363-401

  20. Three-Dimensional Analysis of Single Particles by Electron Microscopy

    • Teresa Ruiz, Michael Radermacher
    Pages 403-425
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Keywords

About this book

A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.

Reviews

From the reviews:

"...deserves to do well." -Ultramicroscopy

"The book covers a wide range of experimental imaging methods for the investigation of cells. … The book is well produced, with many excellent photographs and diagrams, and I have enjoyed broadening my awareness of techniques that not only did I not know in detail but in some cases not even know existed. A nice book … ." (D. J. Cook, British Journal of Biomedical Science, Vol. 63 (2), 2006)

"This 21-chapter volume … provides detailed descriptions of and protocols for a plethora of microscopy and related methods to study tissues, cells and macromolecules. … This comprehensive and well rounded book is suitable for institutional or personal purchase, novices and experts, and it is certainly a must-read for all serious confocal microscopists and imaging facility managers." (Andreas Holzenburg, Microbiology Today, August, 2006)

"Douglas Taatjes and Brooke Mossman have edited a compilation of 21 articles. … The subjects covered are very diverse, ranging from light microscopy to electron microscopy. … In summary, this book provides an excellent overview on current and relevant imaging techniques. The described methods and protocols are not only of particular interest to those who are new in the field or who have to expand their imaging capability above regular light microscopy. The book will serve as a reference for years to come … ." (Carsten Schultz, ChemBioChem, Vol. 7, 2006)

"This text will be extremely useful. … it is a collection of advanced and sometimes disparate microscopy techniques and their application to biological research. … The authors include highly detailed descriptions and instructions on the low-level methods computer programmers use for acquisition, storage, and manipulation of digital image data." (Steven Ruzin, Microscope, Vol. 54, 2006)

Editors and Affiliations

  • Department of Pathology, University of Vermont, Burlington

    Douglas J. Taatjes, Brooke T. Mossman

Bibliographic Information

  • Book Title: Cell Imaging Techniques

  • Editors: Douglas J. Taatjes, Brooke T. Mossman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59259-993-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Softcover ISBN: 978-1-61737-394-7Published: 10 November 2010

  • eBook ISBN: 978-1-59259-993-6Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 490

  • Number of Illustrations: 212 b/w illustrations

  • Topics: Anatomy, Cell Biology, Biochemistry, general

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