Molecular Neurosurgery with Targeted Toxins

1. Front Matter

   Pages i-xi

   PDF

2. Introduction to Molecular Neurosurgery

   • Ronald G. Wiley, Douglas A. Lappi

   Pages 1-8

3. Ribosome-Inactivating Proteins

   • Fiorenzo Stirpe

   Pages 9-29

4. Biochemical, Physiological, and Behavioral Characterizations of the Cholinergic Basal Forebrain Lesion Produced by 192 IgG-Saporin

   • Jerene J. Waite

   Pages 31-58

5. Basal Forebrain Cholinergic Lesion by 192 IgG-Saporin

   • Reinhard Schliebs

   Pages 59-86

6. 192 IgG-Saporin-Induced Partial Cortical Cholinergic Deafferentation as a Model for Determining the Interactions Between Brain Aging and Neurodevelopmental Defects in the Cortical Cholinergic Input System

   • Martin Sarter, John P. Bruno

   Pages 87-100

7. Exploring the Role of Acetylcholine in Primate Cognition Using Me20.4 IgG-Saporin

   • Rosalind M. Ridley, Harry F. Baker

   Pages 101-142

8. Cortical Cholinergic Deafferentation Induces Aβ Deposition

   • Thomas G. Beach, D. G. Walker, P. E. Potter, L. I. Sue, S. Scott, K. J. Layne et al.

   Pages 143-180

9. Chemical Dissection of Brain Glucoregulatory Circuitry

   • Sue Ritter, Thu T. Dinh, Kishor Bugarith, Dawna M. Salter

   Pages 181-218

10. Cardiovascular Deficits After Lesions of C1 Adrenergic Neurons With a Saporin-Based Immunotoxin

    • Patrice G. Guyenet, Ruth L. Stornetta, Ann M. Schreihofer

    Pages 219-233

11. Saporin Conjugates and Pain

    • Ronald G. Wiley, Douglas A. Lappi

    Pages 235-248

12. The Use of Saporin Conjugates to Dissect Neurons Responsible for Sleep and Wakefulness

    • Carlos Blanco-Centurion, Dmitry Gerashchenko, Eric Murillo-Rodriguez, Frank Desarnaud, Priyattam J. Shiromani

    Pages 249-264

13. Isolectin IB4-Mediated Cytotoxic Targeting of Sensory Neurons

    • Lucy Vulchanova, Christopher N. Honda

    Pages 265-291

14. B Fragment of Cholera Toxin Conjugated to Saporin

    • Peter T. Ohara, Kanwarjit Kelley, Luc Jasmin

    Pages 293-306

15. Back Matter

    Pages 307-311

    PDF

Pioneers and leading researchers explain the theory and techniques of using targeted toxins experimentally. The highly successful use of the 192 IgG-saporin and ME20.4-saporin immunotoxins to lesion the cholinergic basal forebrain in order to model the behavior, anatomy, physiology, and pharmacology of Alzheimer's disease in animals is treated in detail to give a potential user the knowledge to comfortably use the techniques involved. The uses of important new lesioning agents such as anti-DBH-saporin immunotoxin to make remarkably selective lesions of catecholaminergic neurons, hypocretin-saporin that can produce narcoleptic animals, and other saporin conjugates, such as neuropeptide-saporin conjugates for pain research and cholera toxin B chain-saporin to produce a model of CNS demyelination are explained by experts in the field.

• Alzheimer
• Alzheimer´s disease
• CNS
• aging
• alzheimer's disease
• anatomy
• brain
• cognition
• forebrain
• neurons
• neurosurgery
• physiology
• protein
• research
• surgery

"...a good purchase for most neuroscience and neurology libraries." - Doody's Health Science Book Review Journal

• Department of Neurology, Vanderbilt University School of Medicine, Nashville

  Ronald G. Wiley

• Department of Pharmacology, Vanderbilt University School of Medicine, Nashville

  Ronald G. Wiley

• Neurology Service, Veterans Affairs Tennessee Valley Healthcare System, Nashville

  Ronald G. Wiley

• Advanced Targeting Systems, San Diego

  Douglas A. Lappi

Policies and ethics